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CBL1346Z-I Anti-mouse Integrin alphaV Antibody, clone RMV-7 (Preservative free)

This Anti-mouse Integrin alphaV Antibody, clone RMV-7 (Preservative free) is validated for use in Western Blotting, Immunoprecipitation, Flow Cytometry for the detection of mouse Integrin alphaV.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: Itgav UniProt Number: P43406
CBL1346Z-I
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWB, IP, FCRPurifiedMonoclonal Antibody
Description
Catalogue NumberCBL1346Z-I
ReplacesCBL1346Z
DescriptionAnti-mouse Integrin alphaV Antibody, clone RMV-7 (Preservative free)
Alternate Names
  • Integrin alpha-V
  • Vitronectin receptor subunit alpha
  • CD51
  • 1.Integrin alpha-V heavy chain2.Integrin alpha-V light chain
  • Integrin alphaV
Background InformationIntegrin alphaV, also known as CD51, a member of the integrin family, is a 140 kD protein expressed on activated T cells, polymorphonuclear granulocytes, blastocysts, and osteoclasts. Integrin alphaV forms heterodimers by association with integrins beta1, beta3, beta5 or beta6, these complexes then act as receptors for multiple extracellular matrix proteins (ECM). The alphaV integrins have varied functions in development, stimulation/activation and homeostasis. The primary ligands for Integrin alphaV complexes are fibronectin, fibrinogen, vitronectin, thrombspondin, von Willebrand factor, and CD31.
References
Product Information
FormatPurified
Control
  • Murine 3T3-L1 cells
PresentationPurified rat monoclonal IgG1κ in buffer containing PBS without preservatives.
Quality LevelMQ100
Applications
ApplicationThis Anti-mouse Integrin alphaV Antibody, clone RMV-7 (Preservative free) is validated for use in Western Blotting, Immunoprecipitation, Flow Cytometry for the detection of mouse Integrin alphaV.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Flow Cytometry
Application NotesWestern Blot Analysis: A representative lot was used by an independent laboratory in LAK cell lysate. (Takahashi, K. et al., (1990). J. Immunol. 145:4371.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in LAK cell lysate. (Takahashi, K. et al., (1990). J. Immunol. 145:4371.)

Blocking Assay: Adhesion and LAK cell cytotoxicity was shown using a representative lot (Takahashi, K. et al., (1990). J. Immunol. 145:4371.)
Biological Information
ImmunogenBALB/c mouse IL-2–activated killer (LAK) cells
CloneRMV-7
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRat
SpecificitySpecifically recognizes Integrin alphaV, also known as CD51 or vitronectin receptor. This antibody has been reported to block binding of Integrin alphaV to vitronectin, fibronectin, fibrinogen, and CD31 in some cell types, as well as, blocking LAK cell cytotoxicity.
IsotypeIgG1κ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Itgav
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~140 kDa observed. The calculated molecular weight is 115 kDa, however Integrin alphaV has been shown as a ~140 kDa band in western blots (Takahashi, K. et al., (1990). J. Immunol. 145:4371.).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in murine 3T3-L1 cells.

Flow Cytometry Analysis: 0.2 µg of this antibody detected Integrin alphaV in murine 3T3-L1 cells .
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CBL1346Z-I 04053252819056

Documentation

Anti-mouse Integrin alphaV Antibody, clone RMV-7 (Preservative free) Certificates of Analysis

TitleLot Number
Anti-mouse Integrin alphaV, clone -2718261 2718261
Anti-mouse Integrin alphaV, clone RMV-7 2472968
Anti-mouse Integrin alphaV, clone RMV-7 (Preservative free) - 3194688 3194688
Anti-mouse Integrin alphaV, clone RMV-7 (Preservative free) - 3463624 3463624
Anti-mouse Integrin alphaV, clone RMV-7 (Preservative free) - 4048706 4048706
Anti-mouse Integrin alphaV, clone RMV-7 (Preservative free) - Q2067202 Q2067202
Anti-mouse Integrin alphaV, clone RMV-7 (Preservative free) Monoclonal Antibody 3031663

References

Reference overviewPub Med ID
A murine very late activationantigen-like extracellular matrix receptor involved in CD2- and lymphocyte function-associated antigen-1-independent killer-targetcell interaction
Takahashi, K. et al.
J. Immunol., 145:4371­4379 (1990)  1990

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