Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABN1480 Anti-p47-phox Antibody

ABN1480
100 μg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN1480
DescriptionAnti-p47-phox Antibody
Alternate Names
  • Neutrophil cytosol factor 1
  • NCF-1
  • 47 kDa neutrophil oxidase factor
  • NCF-47K
  • Neutrophil NADPH oxidase factor 1
  • p47-phox
  • p47-phox
Background InformationNeutrophil cytosol factor 1 (UniProt Q09014), also known as 47 kDa neutrophil oxidase factor, NADPH oxidase subunit, Ncf-1, NCF-47K, Neutrophil NADPH oxidase factor 1, p47-phox, p47phox) is encoded by the Ncf1 gene in murine species. Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors, including p47-phox, p67-phox, p40-phox and Rac. In the resting cell, p47phox, p67phox, and p40phox form a large molecular weight cytosolic complex. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-p47-phox Antibody is an antibody against p47-phox for use in Western Blotting, Immunohistochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected p47-phox in mouse cerebral cortex and mouse small intestine.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of mouse p47-phox.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteMouse, Human. Predicted to react with Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Ncf1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~47 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected p47-phox in 10 µg of Raji cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN1480 04055977169195