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05-636-I Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 is a highly specific mouse monoclonal antibody, that targets Histone H2A.X & has been tested in western blotting, ICC, ChIP & Immunofluorescence.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: H2AFX, H2AX, H2A.X UniProt Number: P16104
05-636-I
200 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, ICC, ChIP, IFMPurifiedMonoclonal Antibody
Description
Catalogue Number05-636-I
DescriptionAnti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301
Alternate Names
  • Histone H2A.x
  • H2a/x
  • H2A.XS139p
  • H2AXS139p
Background InformationThe histone H2A.X protein is a variant member of the H2A family of histones that is distinguished from other H2A histones by a unique carboxy-terminal sequence. This unique sequence is highly conserved throughout eukaryotic evolution and is rapidly phosphorylated by ATM or ATR at the fourth residue from the carboxy-terminus (Serine 139 in mammalian H2A.X) in response to DNA double-strand breaks (DSBs). Phosphorylation of H2A.X is important in the formation of a stable repair complex at the site of DNA damage.
H2A.X phosphorylation is a very rapid response to DNA damage, occurring within as little as one minute after exposure to ionizing radiation. Phosphorylation of H2A.X occurs irrespective of the cause of the DNA DSBs and phospho-H2A.X has been observed in response to environmental stresses that result in DSBs as well as programmed cellular events, including DNA rearrangement and apoptosis.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 is a highly specific mouse monoclonal antibody, that targets Histone H2A.X & has been tested in western blotting, ICC, ChIP & Immunofluorescence.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Chromatin Immunoprecipitation (ChIP)
  • Immunofluorescence
Application NotesImmunocytochemistry Analysis: A 1:50-250 dilution from a representative lot detected phospho-Histone H2A. X (Ser139) in HeLa and A431 cells.
Previous lot has been demonstrated to work in Immunofluorescence and Chromatin Immunoprecipitation: See reference (Meier, Andreas et al., 2007)
Western Blotting Analysis: 0.05-1 μg/mL of this lot detected phosphorylated histone H2A.X (Ser139) in acid extracted histone lysates from Jurkat cells treated with 0.5 μM staurosporine (Catalog # 19-123).
Immunocytochemistry: 2 μg/mL of a previous lot of antibody detected phosphorylated histone H2A.X in HeLa cells treated with 0.5μM staurosporine for 4-6 hours.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human phospho-Histone H2A. X (Ser139).
CloneJBW301
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • H2AFX
  • H2AX
  • H2A.X
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~17 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho-Histone H2A. X (Ser139) in 200 µg in staurosporine treated HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-636-I 04053252934995

Documentation

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 Certificates of Analysis

TitleLot Number
Anti-phospho-Histone H2A.X (Ser139), -2723666 2723666
Anti-phospho-Histone H2A.X (Ser139), -2745891 2745891
Anti-phospho-Histone H2A.X (Ser139), -2794963 2794963
Anti-phospho-Histone H2A.X (Ser139), clone JBW301 2861355
Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 2363579 2363579
Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 2361863 2361863
Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 3185004 3185004
Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 3212740 3212740
Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 3251477 3251477
Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 3279384 3279384
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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies