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70794 BugBuster® GST•Bind™ Purification Kit

Affinity purification of GST fusion proteins

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70794
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
70794-3CN Glass bottle 1 kit
Description
Overview

The BugBuster® GST•Bind™ Purification Kit combines the GST•Bind Resin, GST•Bind Buffer Kit reagents and BugBuster Protein Extraction Reagent for convenient preparation of soluble cell extracts and affinity purification of GST•Tag™ fusion proteins. BugBuster Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E. coli, resulting in the liberation of soluble protein. Cells are harvested by centrifugation as usual, followed by suspension in BugBuster Reagent. During a brief incubation, soluble proteins are released. The extract is clarified by centrifugation, which removes cell debris and insoluble proteins. The clarified extract is ready to apply to GST•Bind Resin.
Catalogue Number70794
Brand Family Novagen®
References
Product Information
•2 x 100 mlBugBuster Protein Extraction Reagent
•10,000 UBenzonase Nuclease, purity > 90%
•10 mlGST•Bind Resin
•pkg/4Chromatography Columns
•2 x 100 ml10X GST Bind/Wash Buffer
•40 ml10X Glutathione Reconstitution Buffer
•1 gGlutathione, Reduced
HS Code3824 99 89
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/38

Irritating to eyes and skin.
S PhraseS: 26

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage Multiple storage requirements
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
70794-3CN 04055977273236

Documentation

BugBuster® GST•Bind™ Purification Kit SDS

Title

Safety Data Sheet (SDS) 

BugBuster® GST•Bind™ Purification Kit Certificates of Analysis

TitleLot Number
70794

Citations

Title
  • Xiaobing Chen, et al. (2008) Organization of the core structure of the postsynaptic density. Procedings of the National Academy of Science 105, 4453-4458.
  • Bryan T. Carroll, et al. (2006) Sulfated signal from ASJ sensory neurons modulates stomatin-dependent coordination in Caenorhabditis elegans. Journal of Biological Chemistry 281, 35989-35996.
  • Laigeng Li, et al. (2005) Clarification of cinnamoyl co-enzyme a reductase catalysis in monolignol biosynthesis of aspen. Plant and Cell Physiology 46, 1073-1082.
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    User Protocols

    Title
    TB235 GST•Bind™ Kits
    TB245 BugBuster® Protein Extraction Reagent