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534350 CDC-like Kinase Inhibitor, Cpd-2 - Calbiochem

A cell-permeable, highly potent, and specific inhibitor of CDC-like kinase (CLK; IC₅₀ = 1.1 and 2.4 nM for CLK1 and CLK2, respectively).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: CLK Inhibitor, Cpd-2

Primary Target: CLK1
534350
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₂₀H₂₀N₆O

Products

Catalogue NumberPackaging Qty/Pack
5.34350.0001 Glass bottle 5 mg
Description
OverviewA cell-permeable imidazo[1,2-b]pyrazol derived compound that acts as a highly potent and specific inhibitor of CDC-like kinases (CLK; IC50 = 1.1 and 2.4 nM for CLK1 and CLK2, respectively). Exhibits only trivial activity against serine-arginine protein kinases (SRPK) 1, 2, and 3 (IC50 = 200, 310, and 230 nM, respectively). Induces pre-mRNA splicing alteration of multiple genes involved in growth and survival pathways resulting in the depletion of respective proteins. Causes a significance decrease in endogenous phosphorylated serine-arginine proteins and enlarges nuclear speckles in MDA-MB-468 cells. Induces apoptosis and Inhibits the growth of multiple cancer cell lines (GI50 = 3.0, 1.9, 1.7, 2.2, 1.4, 2.0, and 0.6 µM for MDA-MB-468, A549, COLO205, HCT116, NCIH23, SW620, and COLO320 DM cells, respectively).
Catalogue Number534350
Brand Family Calbiochem®
SynonymsCLK Inhibitor, Cpd-2
References
ReferencesAraki, S., et al. 2015. Plos One. 10, e0116929.
Product Information
FormLight pink solid
Hill FormulaC₂₀H₂₀N₆O
Chemical formulaC₂₀H₂₀N₆O
ReversibleY
Quality LevelMQ100
Applications
Biological Information
Primary TargetCLK1
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
5.34350.0001 04054839117138

Documentation

CDC-like Kinase Inhibitor, Cpd-2 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Araki, S., et al. 2015. Plos One. 10, e0116929.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision28-July-2017 JSW
SynonymsCLK Inhibitor, Cpd-2
DescriptionA cell-permeable imidazo[1,2-b]pyrazol derived compound that acts as a highly potent and specific inhibitor of CDC-like kinases (CLK; IC50 = 1.1 and 2.4 nM for CLK1 and CLK2, respectively). Exhibits only trivial activity against serine-arginine protein kinases (SRPK) 1, 2, and 3 (IC50 = 200, 310, and 230 nM, respectively). Induces pre-mRNA splicing alteration of multiple genes involved in growth and survival pathways resulting in the depletion of respective proteins. Causes a significance decrease in endogenous phosphorylated serine-arginine proteins and enlarges nuclear speckles in MDA-MB-468 cells. Induces apoptosis and Inhibits the growth of multiple cancer cell lines (GI50 = 3.0, 1.9, 1.7, 2.2, 1.4, 2.0, and 0.6 µM for MDA-MB-468, A549, COLO205, HCT116, NCIH23, SW620, and COLO320 DM cells, respectively).
FormLight pink solid
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₂₀H₂₀N₆O
Purity≥98% by HPLC
SolubilityDMSO (100 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesAraki, S., et al. 2015. Plos One. 10, e0116929.