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SCM017 ENStem-A™ Neuronal Differentiation Medium

The ENStem-A Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of ENStem-A human neural progenitor cells to a neuronal lineage.

ENStem-A™ Neuronal Differentiation Medium MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
SCM017
100 mL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
Cult
Description
Catalogue NumberSCM017
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionENStem-A™ Neuronal Differentiation Medium
OverviewENStem-A™ Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of ENStem-A™ human neural progenitor cells to a neuronal lineage. The medium has been extensively validated on ENStem-A™ Human Neural Progenitor Cells. Using ENStem-A™ Neuronal Differentiation Medium, relatively pure populations of neuronal cells (>90% bIII-tubulin positive) are obtained. Very low levels of astrocytes (<0.5% GFAP positive) are detected. Sufficient medium is supplied to provide for 100 separate reactions at 1 mL volume or 10 reactions at 10 mL volume.
Materials Required but Not Delivered• L-Glutamine (Cat. No. TMS-002-C)
• ENStem-A™ Human Neural Progenitor Cells Kit (Cat. No. SCR055)
• ENStem-A™ Neural Expansion Medium (Cat. No. SCM004)
• ENStem-A™ Neural Freezing Medium (Cat. No. SCM011)
• Human Neural Stem Cell Characterization Kit (Cat. No. SCR060)
• Poly-L-ornithine (Sigma Part No. P3655)
• Laminin (Cat. No. CC095)
• Penicillin/Streptomycin Solution (Cat. No. TMS-AB2-C)
• Accutase™ (Cat. No. SCR005)
• Chamber slides
• Tissue culture-ware
• Phosphate-Buffered Saline (1X PBS) (Cat. No. BSS-1005-B)
• Hemocytometer
• Microscope
References
Product Information
Quality LevelMQ100
Applications
ApplicationThe ENStem-A Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of ENStem-A human neural progenitor cells to a neuronal lineage.
Key Applications
  • Stem Cell Culture
Application NotesCell culture protocols for use with ENStem-A™ Human Neural Progenitor Cells are available in the datasheets that accompany the ENStem-A™ Human Neural Progenitor Expansion Kit (Part No. SCR055).
Biological Information
Media FormLiquid
Stem Cell Type
  • Neural Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEach lot of media is tested for the optimal differentiation of ENStem-A™ Human Neural Progenitor Cells to a neuronal lineage.
Sterility Testing: Negative
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsENStem-A™ Neuronal Differentiation Medium should be stored at -20°C until ready to use. Upon thawing, fresh L-Glutamine (not provided) should be added for a final concentration of 2 mM to the medium. Thawed medium should be stored at 2-8°C and given a 1-month expiration dating. Dispense into aliquots to avoid repeated heating prior to each use.
Packaging Information
Material Size100 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCM017 04053252724527