Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

DC05L Goat Anti-Mouse IgG Alkaline Phosphatase Conjugate

This Goat Anti-Mouse IgG Alkaline Phosphatase Conjugate is validated for use in ELISA Immunoblotting for the detection of Mouse IgG.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
DC05L
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
DC05L-200UGCN Glass bottle 200 μg
Description
Catalogue NumberDC05L
Brand Family Calbiochem®
References
Product Information
FormLyophilized
FormulationLyophilized from 250 mM NaCl, 10 mM Tris-HCl, 15 mg/ml BSA, pH 8.0.
HS Code3002 15 90
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (1:5000-1:100,000)
Immunoblotting (1:5000-1:100,000, colorimetric; 1:10,000-1:200,000, chemiluminescence)
Application CommentsTo ensure the quality of these reagents each lot is tested with several of our primary antibodies. Antibody specificity is tested by immunoelectrophoresis and shown to be specific for mouse IgG and light chains common to most mouse immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins has been detected. The antibody has also been tested by ELISA and/or adsorbed to ensure minimal cross-reactivity with human, bovine, and horse serum proteins, but the antibody may exhibit cross-reactivity with immunoglobulins from other species. Antibody should be titrated for optimal results in individual systems.
Biological Information
ImmunogenMouse
HostGoat
IsotypeIgG
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the antibody with 1 ml H₂O. Dissolve by gently mixing at 4°C, since extensive mixing will denature antibodies. Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Alternatively, glycerol (to a final concentration of 50%) may be added and the reconstituted antibody then stored at -20°C. Please note, under these conditions the concentration of the antibody and the buffer salts will be one-half of the original concentration.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DC05L-200UGCN 07790788060183

Documentation

Goat Anti-Mouse IgG Alkaline Phosphatase Conjugate SDS

Title

Safety Data Sheet (SDS) 

Goat Anti-Mouse IgG Alkaline Phosphatase Conjugate Certificates of Analysis

TitleLot Number
DC05L
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-August-2007 JSW
ApplicationELISA (1:5000-1:100,000)
Immunoblotting (1:5000-1:100,000, colorimetric; 1:10,000-1:200,000, chemiluminescence)
DescriptionImmunoaffinity purified goat polyclonal antibody adsorbed against human, bovine, and horse serum proteins and conjugated to alkaline phosphatase. Recognizes mouse IgG.
HostGoat
Immunogen speciesMouse
IsotypeIgG
FormLyophilized
FormulationLyophilized from 250 mM NaCl, 10 mM Tris-HCl, 15 mg/ml BSA, pH 8.0.
Preservative≤0.1% sodium azide
CommentsTo ensure the quality of these reagents each lot is tested with several of our primary antibodies. Antibody specificity is tested by immunoelectrophoresis and shown to be specific for mouse IgG and light chains common to most mouse immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins has been detected. The antibody has also been tested by ELISA and/or adsorbed to ensure minimal cross-reactivity with human, bovine, and horse serum proteins, but the antibody may exhibit cross-reactivity with immunoglobulins from other species. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the antibody with 1 ml H₂O. Dissolve by gently mixing at 4°C, since extensive mixing will denature antibodies. Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Alternatively, glycerol (to a final concentration of 50%) may be added and the reconstituted antibody then stored at -20°C. Please note, under these conditions the concentration of the antibody and the buffer salts will be one-half of the original concentration.
Toxicity Standard Handling