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234374 Granulocyte Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli

Granulocyte Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli, is a pleiotropic cytokine that can stimulate the proliferation, maturation, and function of hematopoietic cells.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rmGM-CSF

234374
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
234374-10UGCN Plastic ampoule 10 μg
Description
OverviewRecombinant, mouse granulocyte macrophage colony-stimulating factor expressed in E. coli. Pleiotropic cytokine that can stimulate the proliferation, maturation, and function of hematopoietic cells. Reported to improve the response rate to antibiotic therapy in cancer subjects. Activates JAK2 in a variety of cells.
Catalogue Number234374
Brand Family Calbiochem®
SynonymsrmGM-CSF
References
ReferencesAnaissie, E.J., et al. 1996. Am. J. Med. 100, 15.
Watanabe, S., et al. 1996. J. Biol. Chem. 271, 12681.
Moore, M.A. 1991. Annu. Rev. Immunol. 9, 159.
Nicola, N.A. 1989. Annu. Rev. Biochem. 58, 45.
Gough, N.M., et al. 1985. EMBO J. 4, 645.
Product Information
FormLyophilized
FormulationLyophilized from sterile-filtered PBS containing 50 µg BSA/µg GM-CSF.
Quality LevelMQ100
Applications
ApplicationGranulocyte Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli, is a pleiotropic cytokine that can stimulate the proliferation, maturation, and function of hematopoietic cells.
Biological Information
Biological activityED₅₀ = 100-300 pg/ml as measured in a cell proliferation assay using a factor-dependent cell line, DA-3
Purity>97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1.0 EU/µg GM-CSF
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 3 months at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
234374-10UGCN 04055977200133

Documentation

Granulocyte Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Granulocyte Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli Certificates of Analysis

TitleLot Number
234374

References

Reference overview
Anaissie, E.J., et al. 1996. Am. J. Med. 100, 15.
Watanabe, S., et al. 1996. J. Biol. Chem. 271, 12681.
Moore, M.A. 1991. Annu. Rev. Immunol. 9, 159.
Nicola, N.A. 1989. Annu. Rev. Biochem. 58, 45.
Gough, N.M., et al. 1985. EMBO J. 4, 645.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision29-April-2008 RFH
SynonymsrmGM-CSF
DescriptionRecombinant, mouse granulocyte macrophage colony-stimulating factor expressed in E. coli. Pleiotropic cytokine that stimulates the proliferation, maturation, and function of hematopoietic cells. Reported to improve the response rate to antibiotic therapy in cancer subjects. Activates JAK2 in a variety of cells. Target cells include macrophages, granulocytes, and eosinophils. GM-CSF is produced by many cell types, including endothelial cells, fibroblasts, and T-lymphocytes. It can be extracted from most tissues in concentrations exceeding that of serum levels. While many cells produce GM-CSF constitutively, levels of this lymphokine increase rapidly in response to endotoxin, phorbol esters, and certain antigens.
FormLyophilized
FormulationLyophilized from sterile-filtered PBS containing 50 µg BSA/µg GM-CSF.
Purity>97% by SDS-PAGE
ContaminantsEndotoxin: ≤1.0 EU/µg GM-CSF
Biological activityED₅₀ = 100-300 pg/ml as measured in a cell proliferation assay using a factor-dependent cell line, DA-3
SolubilityReconstitute to a concentration of ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 3 months at 4°C.
Toxicity Standard Handling
Merck USA index14, 4538
ReferencesAnaissie, E.J., et al. 1996. Am. J. Med. 100, 15.
Watanabe, S., et al. 1996. J. Biol. Chem. 271, 12681.
Moore, M.A. 1991. Annu. Rev. Immunol. 9, 159.
Nicola, N.A. 1989. Annu. Rev. Biochem. 58, 45.
Gough, N.M., et al. 1985. EMBO J. 4, 645.