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CKT2MAG-97K MILLIPLEX® Canine Kidney Toxicity Panel 2 - Toxicity Multiplex Assay

The analytes available for this multiplex kit are: Albumin, β-2-microglobulin (B2M), Trefoil Factor 3 (TFF-3), Retinol Binding Protein 4 (RBP4)

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Toxicity Research Sub-Category: Toxicity
CKT2MAG-97K
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Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Albumin β-2-Microglobulin Trefoil Factor 3 (TFF-3) Retinol Binding Protein 4 (RBP4) CaMplexLuminex xMAP
Description
Catalogue NumberCKT2MAG-97K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Canine Kidney Toxicity Panel 2 - Toxicity Multiplex Assay
OverviewThe MILLIPLEX® Canine Kidney Toxicity Panel 2 contains all the components necessary to measure the following four biomarkers in any combination using Luminex® xMAP® technology: Albumin, β-2-microglobulin (B2M), Trefoil Factor 3 (TFF-3), Retinol Binding Protein 4 (RBP4). The kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 urine samples in duplicate.
Background InformationAs one of the leading causes of drug candidate attrition, toxicity research is receiving more attention, especially in the search of sensitive, organ-specific toxicity biomarkers.

In pharmaceutical and chemical industries, renal function is routinely assessed during toxicity evaluations. The importance of the kidney as a central detoxification organ leads to a high exposure of renal tissue to drugs, reactive metabolites, and environmental compounds. Traditional markers for assessing renal toxicity, such as blood urea nitrogen (BUN) and serum creatinine, are insensitive and non-specific. For improved, non-invasive detection of acute nephrotoxicity, a panel of novel urinary kidney biomarkers has been identified and validated for preclinical studies in relevant animal species.

EMD Millipore provides a valuable research assay to investigate multiple biomarkers of kidney injury in canine urine samples using the Luminex xMAP® instrument platform. The MILLIPLEX® Canine Kidney Toxicity Panel 2 offers simultaneous analysis of any combination of the following analytes: Albumin*, β-2-microglobulin (B2M)*, Trefoil Factor 3 (TFF-3)*, Retinol Binding Protein 4 (RBP4)*.

*Urinary albumin, B2M, TFF-3 and RBP4 are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing.
References
Product Information
Components
  • Canine Kidney Toxicity Panel 2 Standard, 1 vial, lyophilized
  • Canine Kidney Toxicity Panel 2 Control 1, 1 vial, lyophilized
  • Canine Kidney Toxicity Panel 2 Control 2, 1 vial, lyophilized
  • Assay Buffer, 2 bottles, 30 mL
  • Wash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mL
  • Canine Kidney Toxicity Panel 2 Detection Antibodies, 1 bottle, 5.5 mL
  • Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
  • Mixing Bottle, 1 bottle
  • Sample Plate, 96 well format, 1 plate
  • 2 Foil Plate Sealers
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: <15
  • Intra-assay: <10
Panel TypeMAGNETIC Toxicity
Quality LevelMQ200
Applications
ApplicationThe analytes available for this multiplex kit are: Albumin, β-2-microglobulin (B2M), Trefoil Factor 3 (TFF-3), Retinol Binding Protein 4 (RBP4)
Key Applications
  • Multiplexing
Application Notes• Customers should determine the optimal dilution factor for their samples. Our recommendations are based on urine samples from normal subjects.
• This assay requires 25 µL of 1:500 diluted urine sample per well in kit Assay Buffer (please dilute per protocol instructions).
• This is a 2 hour assay.
Biological Information
SpecificityApproximately 5% cross-reactivity may be observed between B2M beads and TFF-3 protein. No other significant cross-reactivity was observed for other analytes in this panel.
Species Reactivity
  • Canine
Analytes Available
  • Albumin
  • β-2-Microglobulin
  • Trefoil Factor 3 (TFF-3)
  • Retinol Binding Protein 4 (RBP4)
Assay PanelEverything you need in a single kit.
Cross ReactivityApproximately 5% cross-reactivity may be observed between B2M beads and TFF-3 protein. No other significant cross-reactivity was observed for other analytes in this panel.
Accuracy
  • 96% - 102%
Physicochemical Information
Sensitivity
  • Albumin: 12.48 ng/mL (MinDC +2SD)
  • All other analytes: 0.016 - 0.026 ng/mL (MinDC +2SD)
  • See kit protocol for individual analyte sensitivities.
Linearity of DilutionContact technical support for this information.
Standard Curve Range
  • Albumin: 12.21 – 50,000 ng/mL
  • B2M: 0.02 – 100 ng/mL
  • TFF-3: 0.02 – 100 ng/mL
  • RBP4: 0.01 – 50 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CKT2MAG-97K 04055977122565

Documentation

MILLIPLEX® Canine Kidney Toxicity Panel 2 - Toxicity Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
NPI Flyer- Epigenetics and Nuclear Function Feature
New Products - Antibodies, Small Molecule, Inhibitors
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Quality Control Range Sheet- CKT2-106 and CKT2-206
Quality Control Range Sheet- CKT2-108 and CKT2-208
Quality Control Ranges: Milliplex Canine Kidney Toxicity Magnetic Bead Panel 2
White Paper: Further considerations of antibody validation and usage.

Posters

Title
Toxicology in drug discovery and development: Biomarkers and model organisms