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HCYTB-60K MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel B

Simultaneously analyze up to 48 cytokine, chemokine, and growth factor biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

HCYTB-60K
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Overview

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Description
Catalogue NumberHCYTB-60K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel B
OverviewMILLIPLEX® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the verification process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability).

Each MILLIPLEX® panel and kit includes:
• Quality controls (QCs) to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Optimized serum matrix to mimic native analyte environment in serum and plasma samples
• Detection antibody cocktails designed to yield consistent analyte profiles within panel

In addition each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility. The MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel B thus enables you to focus on the therapeutic potential of cytokines and the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
Background Information“Cytokine” is a general term used for a diverse group of small proteins, peptides or glycoproteins secreted by lymphocytes, monocytes, macrophages and other cells that regulate immune responses, hematopoiesis and lymphocyte development. Cytokines include interleukins, interferons, chemokines and other signaling molecules. Growth factors are extracellular polypeptides that have a positive effect on cell growth and proliferation. They can stimulate growth in a variety of different cell types. Growth factors and cytokines are similar in structure and the way of action. Individual cytokines or growth factors are produced by multiple cell types. This is different from hormones, which tend to be made by specialized glands. Each cytokine or growth factor acts through its own receptor on target cells to generate signaling pathways, and as a consequence to regulate biological processes. Some intracellular signaling components are shared between cytokines and growth factors. Expression of cytokines or growth factors and their receptors is highly regulated. De-regulation may contribute to many diseases such as infectious disease, autoimmune and chronic inflammatory disease, cardiovascular disease, metabolic syndrome, neurological disorders, and cancer. Cytokine and growth factor research plays a significant role in achieving a deeper understanding of the immune system and combating related diseases.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay: <15%
  • Inter-assay: <20%
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationSimultaneously analyze up to 48 cytokine, chemokine, and growth factor biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight or two-hour incubation assay.
• This assay requires 25 µL of neat plasma or serum, or 25 μL cell culture supernatant per well.
• This kit may be used for the analysis of any or all of the Human Cytokine/Chemokine/Growth Factor Panel B analytes in human tissue/cell lysate and culture supernatant samples and serum or plasma samples.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • 6Ckine
  • sCD137
  • sFas
  • sFasL
  • APRIL/TNFSF13
  • BAFF/Blys
  • BCA-1
  • CCL28
  • CTACK
  • CXCL16
  • ENA-78
  • Eotaxin-2
  • Eotaxin-3
  • GCP2
  • Granzyme A
  • Granzyme B
  • HMGB1
  • I-309
  • IFNβ
  • IFNω
  • IL-11
  • IL-16
  • IL-20
  • IL-21
  • IL-23
  • IL-24
  • IL-28A
  • IL-29
  • IL-31
  • IL-33
  • IL-34
  • IL-35
  • I-TAC
  • LIF
  • Lymphotactin
  • MCP-2
  • MCP-4
  • MIP-1d
  • MIP-3α
  • MIP-3β
  • MPIF-1/CCL23
  • Perforin
  • SCF
  • SDF-1
  • TARC
  • TPO
  • TRAIL
  • TSLP
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Accuracy
  • 81% - 99%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities for individual cytokines, chemokines and growth factors.
Standard Curve Range
  • 6Ckine: 37-150,000 pg/mL
  • BCA-1, MCP-4: 1.7-7,000 pg/mL
  • sFas: 0.3-1,100 ng/mL
  • sFasL, IL-33: 20-80,000 pg/mL
  • CTACK: 3.7-15,000 pg/mL
  • CXCL16, Eotaxin-3, IL-21, IL-28A: 4.9-20,000 pg/mL
  • ENA-78: 3.1-12,500 pg/mL
  • GCP-2: 0.9-3,500 pg/mL
  • sCD137, APRIL, Granzyme A, Eotaxin-2, IFNβ, IFNω, IL-11, IL-31, LIF, TRAIL: 2.4-10,000 pg/mL
  • BAFF: 13-55,000 pg/mL
  • IL-16, IL-34, MIP-1δ: 10-40,000 pg/mL
  • CCL28: 61-250,000 pg/mL
  • IL-35, SCF, SDF-1, TPO: 12-50,000 pg/mL
  • HMGB1: 134-550,000 pg/mL
  • I-309: 1.5-6,000 pg/mL
  • Granzyme B: 1.8-7,500 pg/mL
  • IL-23, IL-24: 24-100,000 pg/mL
  • IL-20: 15-60,000 pg/mL
  • IL-29: 6.1-25,000 pg/mL
  • Lymphotactin: 7.3-30,000 pg/mL
  • MCP-2: 0.2-1,000 pg/mL
  • MIP-3α: 0.6-2,500 pg/mL
  • I-TAC, MIP-3β: 1.2-5,000 pg/mL
  • MPIF-1: 1.0-4,000 pg/mL
  • TARC: 0.8-3,250 pg/mL
  • TSLP: 0.5-2,000 pg/mL
  • Perforin: 18-75,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HCYTB-60K 04065268025796

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