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48-614MAG MILLIPLEX MAP TGFβ Signaling Pathway Magnetic Bead 6-Plex - Cell Signaling Multiplex Assay

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Signaling
48-614MAG
96-well plate  96-well plate
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Akt (Ser473) ERK (Thr185/Tyr187) Smad2 (Ser465/Ser467) Smad3 (Ser423/Ser425) Smad4 (Total) TGFβRII (Total) HMplexLuminex xMAP
Description
Catalogue Number48-614MAG
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP TGFβ Signaling Pathway Magnetic Bead 6-Plex - Cell Signaling Multiplex Assay
OverviewCells respond to their environment in many different ways through intracellular signaling. The TGFβ pathway plays a central role in a number of normal cellular processes including proliferation, differentiation, apoptosis, migration, adhesion, immune response and other functions in most cells. Although the TGFβ pathway regulates a wide range of processes, the pathway is fairly simple. TGFβ dimers bind to TGFβ Type II Receptor which recruits and phosphorylates TGFβ Type I Receptor. The Type I Receptor then recruits and phosphorylates SMAD2/3, which then binds to SMAD4 and forms a complex that enters the cell nucleus where it acts as a transcription factor for various genes. TGFβ Receptor activates the SMAD-dependent canonical pathway, as well as SMAD-independent non-canonical pathways, such as PI3K/Akt, ERK, JNK, and p38.

Aberrant TGFβ signaling is involved in the development of multiple diseases, including hematological malignancies such as leukemia, and impaired wound healing, neurodegenerative diseases, developmental disorders and pulmonary hypertension. Loss-of-function mutations promote tumorigenesis by suppressing the immune system and the epithelial mesenchymal transition (EMT), although TGFβ signaling has also been implicated in tumor inhibition. Immune cells such as B cells, T cells, dendritic cells and macrophages secrete TGFβ, which downregulates their proliferation, differentiation and activation by other cytokines. Consequently, modifications in TGFβ signaling have been linked to autoimmune and inflammatory diseases and cancer.
References
Product Information
Detection methodLuminex xMAP
ConfigurationPremixed
Precision, %
  • Intra-Assay: < 8
  • Inter-Assay: < 12
Panel TypeCell Signaling
Quality LevelMQ200
Applications
ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
Key Applications
  • Multiplexing
Application Notes• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 1 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • Akt (Ser473)
  • ERK (Thr185/Tyr187)
  • Smad2 (Ser465/Ser467)
  • Smad3 (Ser423/Ser425)
  • Smad4 (Total)
  • TGFβRII (Total)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
48-614MAG 04053252688164

Documentation

Protocols

Title
Protocol- 48-614MAG

MILLIPLEX MAP TGFβ Signaling Pathway Magnetic Bead 6-Plex - Cell Signaling Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression
Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single

Posters

Title
Biological Evaluation of Human PBMC and Th17 cells using MILLIPLEX®MAP Cell Signaling and Cytokine Panels
Cellular Analysis Using MILLIPLEX® MAP TGF-β Signaling Pathway Panel
MILLIPLEX®MAP Cell Signaling Wall Chart