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03-311 Magna ChIRP Mouse XIST lncRNA Probe Set

The Magna ChIRP Mouse XIST lncRNA Probe Set contains 43 predesigned 20-mer DNA oligonucleotides tiled along and complementary to the sequence of mouse lncRNA XIST.

03-311
12 reactions/pack  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number03-311
Brand Family Chemicon®
Trade Name
  • Magna ChIRP
DescriptionMagna ChIRP Mouse XIST lncRNA Probe Set
OverviewChIRP (Chromatin Isolation by RNA Purification), allows recovery of specific segments of DNA and proteins using chromatin associated RNA as a target for biotinylated antisense probes. The Magna ChIRP Mouse XIST lncRNA Probe Set contains 43 predesigned 20-mer DNA oligonucleotides tiled along and complementary to the sequence of mouse lncRNA XIST. All probes are biotin-TEG-labeled at the 3-prime end. The oligonucleotides are split to two pools (even and odd) and are supplied ready for use in the ChIRP method. This probe set is fully compatible with the Maga ChIRP kits (cat. # 17-10494 and 17-10495).
Background InformationPervasive genomic transcription and the role of non-coding RNAs in this process is a rapidly evolving field that remains to be thoroughly explored in gene regulation.
Historically, chromatin immunoprecipitation (ChIP) has been used to interrogate association of proteins with genomic DNA sequences. Given that chromatin contains not only DNA and DNA-binding proteins, but also RNA-binding proteins and RNA, new methods have been developed to map the association of these RNA molecules to distinct regions of the genome. One such method, designated ChIRP (Chromatin Isolation by RNA Purification), allows recovery of specific segments of DNA and proteins using chromatin associated RNA as a target. This unique approach allows isolation of chromatin-associated RNAs (e.g. lncRNAs) within specific regions of genomic DNA sequences, as well as proteomic analysis of protein components of the lncRNA regulatory complexes. The Magna ChIRP™ RNA Interactome Kits (cat. # 17-10494 and 17-10495) facilitate the use of this method and has been proven compatible with this probe set.
X inactivation is an early developmental process in mammalian females that transcriptionally silences one of the pair of X chromosomes, thus providing dosage equivalence between males and females. The process is regulated by several factors, including a region of chromosome X called the X inactivation center (XIC), which encodes the lncRNA XIST (X-inactive specific transcript). Reactivation of both X chromosomes XaXa, via XIST epigenetic silencing, has been identified as a hallmark of primed to naïve stem cell conversion. Recently, using ChIRP–MS, Chu and colleagues captured 81 XIST-bound proteins in four different types of mouse embryonic stem cells during X chromosome inactivation.
References
Product Information
Presentation10 mM Tris-HCl, 1mM EDTA, pH 8.0
Quality LevelMQ100
Applications
ApplicationThe Magna ChIRP Mouse XIST lncRNA Probe Set contains 43 predesigned 20-mer DNA oligonucleotides tiled along and complementary to the sequence of mouse lncRNA XIST.
Key Applications
  • DNA-binding Proteins
  • RNA Detection
  • DNA Sequencing
  • Protein Determination
  • Protein Interaction Assays
  • Purification with magnetic beads
Biological Information
ConcentrationPlease refer to lot specific datasheet.
Entrez Gene Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsUpon receipt store at -20°C. Avoid multiple freeze thaws. With proper storage and handling this product is stable for 6 months from the date of receipt.
Packaging Information
Material Size12 reactions/pack
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
03-311 04054839317934

Documentation

Magna ChIRP Mouse XIST lncRNA Probe Set SDS

Title

Safety Data Sheet (SDS) 

Magna ChIRP Mouse XIST lncRNA Probe Set Certificates of Analysis

TitleLot Number
Magna ChIRP™ Mouse XIST lncRNA Probe Set Q2925014
Magna ChIRP™ Mouse XIST lncRNA Probe Set - 3194178 3194178
Magna ChIRP™ Mouse XIST lncRNA Probe Set - 3506795 3506795
Magna ChIRP™ Mouse XIST lncRNA Probe Set - 3543977 3543977
Magna ChIRP™ Mouse XIST lncRNA Probe Set - 4033845 4033845
Magna ChIRP™ Mouse XIST lncRNA Probe Set - 4293950 4293950
Magna ChIRP™ Mouse XIST lncRNA Probe Set -3762773 3762773

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