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03-307 Magna ChIRP™ Negative Control Probe Set

The Magna ChIRP Negative Control Probe Set contains 48 predesigned 20-mer DNA oligonucleotides tiled along and complementary to the mRNA sequence of lacZ (β-galactosidase) and is used as a negative control for ChIRP experiments.

Magna ChIRP™ Negative Control Probe Set MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
03-307
12 reactions   
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number03-307
Trade Name
  • Magna ChIRP
DescriptionMagna ChIRP™ Negative Control Probe Set
OverviewChIRP (Chromatin Isolation by RNA Purification), allows recovery of specific segments of DNA and proteins using chromatin associated RNA as a target for biotinylated antisense probes. The Magna ChIRP Negative Control Probe Set contains 48 predesigned 20-mer DNA oligonucleotides tiled along and complementary to the mRNA sequence of Escherichia coli lacZ (β-galactosidase). All probes are biotin-TEG-labeled at the 3-prime end. Because LacZ gene is normally absent from mammalian cells, this primer set is ideal for use as negative control when performing ChIRP experiments with mammalian cells. This probe set is fully compatible with the Maga ChIRP kits (cat. # 17-10494 and 17-10495).
Materials Required but Not DeliveredMagna ChIRP™ RNA Interactome Kit, cat# 17-10494 or EZ-Magna ChIRP™ RNA Interactome Kit, cat# 17-10495
References
Product Information
Presentation50 µM biotin-TEG-labeled 20-mer DNA oligonucleotides in containing 10 mM Tris-HCl, 1mM EDTA, pH 8.0
Quality LevelMQ100
Applications
ApplicationThe Magna ChIRP Negative Control Probe Set contains 48 predesigned 20-mer DNA oligonucleotides tiled along and complementary to the mRNA sequence of lacZ (β-galactosidase) and is used as a negative control for ChIRP experiments.
Key Applications
  • DNA-binding Proteins
  • RNA Detection
  • DNA Sequencing
  • Protein Determination
  • Protein Interaction Assays
  • Purification with magnetic beads
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsUpon receipt store at -20°C. Avoid multiple freeze thaws. With proper storage and handling this product is stable for 6 months from the date of receipt.
Packaging Information
Material Size12 reactions
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
03-307 04055977123531

Documentation

Magna ChIRP™ Negative Control Probe Set SDS

Title

Safety Data Sheet (SDS) 

Magna ChIRP™ Negative Control Probe Set Certificates of Analysis

TitleLot Number
Magna ChIRP™ Negative Control Probe Set 3085847
Magna ChIRP™ Negative Control Probe Set 2914990
Magna ChIRP™ Negative Control Probe Set - 3182317 3182317
Magna ChIRP™ Negative Control Probe Set - 3474328 3474328
Magna ChIRP™ Negative Control Probe Set - 3488580 3488580
Magna ChIRP™ Negative Control Probe Set - 3537193 3537193
Magna ChIRP™ Negative Control Probe Set - 3537772 3537772
Magna ChIRP™ Negative Control Probe Set - 3608701 3608701
Magna ChIRP™ Negative Control Probe Set - 3700531 3700531
Magna ChIRP™ Negative Control Probe Set - 3744421 3744421
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Brochure

Title
Magna ChIRP™ RNA Interactome Kits
NPI Flyer- Epigenetics and Nuclear Function Feature
New Products - Antibodies, Small Molecule, Inhibitors

Technical Info

Title
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics
White Paper: Further considerations of antibody validation and usage.

Posters

Title
Analysis of Long Noncoding RNA Chromatin Interactions by ChIRP