Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

FCMAB186F Milli-Mark Anti-CD20 -FITC Antibody, clone B-Ly1

This Milli-Mark Anti-CD20 -FITC Antibody, clone B-Ly1 is validated for use in FC for the detection of CD20.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology UniProt Number: P11836
FCMAB186F
100 tests  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMFITCMonoclonal Antibody
Description
Catalogue NumberFCMAB186F
Trade Name
  • Milli-Mark
DescriptionMilli-Mark Anti-CD20 -FITC Antibody, clone B-Ly1
Alternate Names
  • Membrane-spanning 4-domains subfamily A member 1
    B-lymphocyte surface antigen B1
    Leukocyte surface antigen Leu-16
    Bp35
    CD_antigen=CD20
Background InformationCD20 is a transmembrane, non-glycosylated protein expressed on B-cell precursors and mature B cells, but is lost following differentiation into plasma cells (Tedder, 1994). In resting B cells, CD20 appears in a 33 kDa non-phosphorylated form. After mitogen stimulation, CD20 becomes heavily phosphorylated (35-37 kDa isoforms), and it is a dominant phosphoprotein in activated B cells and B-cell lines (Zhou, 1995). The long N- and C-terminal ends of the protein are located on the cytoplasmic side of the membrane and only a minor portion of the protein is exposed on the cell surface (Tedder, 1994). Antibodies reacting with CD20 cytoplasmic epitopes are designated CD20cy (Zhou, 1995). It is suggested that CD20 plays a direct role in regulating the transmembrane conductive Ca 2+ flux of B cells which indicates a possible function for CD20 as a regulator of proliferation and differentiation (Tedder, 1994).
References
Product Information
FormatFITC
Control
  • Human Lymphocytes
PresentationPurified Mouse monoclonal IgG1k conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2
Quality LevelMQ100
Applications
ApplicationThis Milli-Mark Anti-CD20 -FITC Antibody, clone B-Ly1 is validated for use in FC for the detection of CD20.
Key Applications
  • Flow Cytometry
Biological Information
CloneB-Ly1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityAntibody recognizes Human CD20 antigen.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight 33 kDa (Zhou, 1995)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by flow cytometry using human lymphocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size100 tests
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
FCMAB186F 04053252289149

Documentation

Milli-Mark Anti-CD20 -FITC Antibody, clone B-Ly1 SDS

Title

Safety Data Sheet (SDS) 

Milli-Mark Anti-CD20 -FITC Antibody, clone B-Ly1 Certificates of Analysis

TitleLot Number
Milli-Mark Anti-CD20 -FITC, clone B-Ly1 - 2080325 2080325
Milli-Mark Anti-CD20 -FITC, clone B-Ly1 - 2199657 2199657
Milli-Mark Anti-CD20 -FITC, clone B-Ly1 - NRG1768290 NRG1768290
Milli-Mark™ Anti-CD20 -FITC, -2523211 2523211
Milli-Mark™ Anti-CD20 -FITC, -2717661 2717661
Milli-Mark™ Anti-CD20 -FITC, clone B-Ly1 - 4087579 4087579
Milli-Mark™ Anti-CD20 -FITC, clone B-Ly1 Conjugate 3135431
Milli-Mark™ Anti-CD20 -FITC, clone B-Ly1 - 2390566 2390566
Milli-Mark™ Anti-CD20 -FITC, clone B-Ly1 - 2397832 2397832
Milli-Mark™ Anti-CD20 -FITC, clone B-Ly1 - 2439672 2439672
Show more

Related Products & Applications

Related Products

Catalogue Number Description
MAB1217-AF647 Anti-CD20 Antibody, clone FMC7, Alexa Fluor® 647 Conjugate
MABF1635 Anti-CD20 (human), PE, clone 2H7 Antibody
MABF1636 Anti-CD20 (human), PE, clone 2H7 Antibody
MABF1637 Anti-CD20 (human), PE, clone 2H7 Antibody
MABF1638 Anti-CD20 (human), violetFluor™ 450, clone 2H7 Antibody
MABF1639 Anti-CD20 (human), violetFluor™ 450, clone 2H7 Antibody
MABF1640 Anti-CD20 (human), violetFluor™ 450, clone 2H7 Antibody
MABF1641 Anti-CD20 (human), redFluor™ 710, clone 2H7 Antibody
MABF1642 Anti-CD20 (human), redFluor™ 710, clone 2H7 Antibody
MABF1643 Anti-CD20 (human), redFluor™ 710, clone 2H7 Antibody

Product Families

Flow Cytometry Kits & Antibodies

Merck's Milli-Mark and FlowCellect brands include fluorescent conjugated antibodies and multiparameter kits for cellular analysis. Designed, optimized, and validated for flow.
Learn More >>

Related Products By: Brand Facete

Milli-Mark™

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies