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MAC506-100UG Mouse Anti-Llama IgG3 Antibody, clone 8E1

Mouse Anti-Llama IgG3, clone 8E1, Cat. No. MAC506, is a mouse monoclonal antibody that detects Llama IgG3 and is tested for use in ELISA, Immunoaffinity, Purification, and Western Blotting.

MAC506-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMAC506-100UG
DescriptionMouse Anti-Llama IgG3 Antibody, clone 8E1
Alternate Names
  • IgG3 HCAb
  • IgG3 Nanobody
Background InformationLlama, camels, and alpacas are camelids that produce normal IgG1 like antibodies and also functional IgG isotypes that do not incorporate light chains and possess only heavy chains. These heavy-chain antibodies may constitute about 50% of the IgG in llama serum and as much as 75% of the IgG in camel serum. By virtue of their smaller size they exhibit better bio-distribution, tissue penetration, and monomeric behavior. IgG3 consists of dimers of short heavy chains, which are characterized by a normal Fc region without a CH1 domain. Although it lacks the CH1 region, it still retains an antigen binding domain called the VHH region. Clone 8E1 is shown to specifically bind to IgG3 and not to any of the IgG2 heavy chains. (Ref.: Daley, LP., et al. (2005). Clin. Diag. Lab. Immunol. 12(4); 380-386).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationMouse Anti-Llama IgG3, clone 8E1, Cat. No. MAC506, is a mouse monoclonal antibody that detects Llama IgG3 and is tested for use in ELISA, Immunoaffinity, Purification, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoaffinity Purification
  • ELISA
Application NotesTested Applications

Immunoaffinity Purification: A representative lot was used in Immunoaffinity Purification applications (Daley, L.P., et. al. (2005). Clin Diagn Lab Immunol. 12(3):380-6; Daley-Bauer, L.P., et. al. (2010). Clin Vaccine Immunol. 17(12):2007-15).

ELISA Analysis: A representative lot detected Mouse Anti-Llama IgG3 in ELISA applications (Daley, L.P., et. al. (2005). Clin Diagn Lab Immunol. 12(3):380-6; Daley-Bauer, L.P., et. al. (2010). Clin Vaccine Immunol. 17(12):2007-15).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenPurified Llama serum IgG3.
EpitopeUnknown
Clone8E1
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 8E1 is a mouse monoclonal antibody that detects Llama IgG3.
Species Reactivity
  • Mammals
Species Reactivity NoteLlama.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
UniProt Number
Molecular Weight~47 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with IgG3.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Llama IgG3, but not IgG1 and IgG2.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAC506-100UG 04065266298437

Documentation

Mouse Anti-Llama IgG3 Antibody, clone 8E1 Certificates of Analysis

TitleLot Number
Mouse Anti-Llama IgG3, clone 8E1 - 3899617 3899617
Mouse Anti-Llama IgG3, clone 8E1 - 3931791 3931791
Mouse Anti-Llama IgG3, clone 8E1 - 3985009 3985009
Mouse Anti-Llama IgG3, clone 8E1 - 4010114 4010114
Mouse Anti-Llama IgG3, clone 8E1 - 4026474 4026474
Mouse Anti-Llama IgG3, clone 8E1 - 4035509 4035509
Mouse Anti-Llama IgG3, clone 8E1 - 4035834 4035834
Mouse Anti-Llama IgG3, clone 8E1 - 4070802 4070802
Mouse Anti-Llama IgG3, clone 8E1 - 4097754 4097754
Mouse Anti-Llama IgG3, clone 8E1 - 4156827 4156827
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