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14-736 TAO2 Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, TAO2. TAO2 is a mitogen-activated protein kinase kinase kinase (MAP3K) that doubly phosphorylates & activates the MAP kinase kinases (MAP2Ks) MEK3 & MEK6. For use in Kinase Assays.

TAO2 Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-736
10 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-736
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionTAO2 Protein, active, 10 µg
OverviewN-terminal 6His-tagged, recombinant, TAO2. TAO2 is a mitogen-activated protein kinase kinase kinase (MAP3K) that doubly phosphorylates and activates the MAP kinase kinases (MAP2Ks) MEK3 and MEK6.
References
Product Information
Presentation10 μg of enzyme in 100 μL of 50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Quality LevelMQ100
Applications
ApplicationActive, N-terminal 6His-tagged, recombinant, TAO2. TAO2 is a mitogen-activated protein kinase kinase kinase (MAP3K) that doubly phosphorylates & activates the MAP kinase kinases (MAP2Ks) MEK3 & MEK6. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
PurityPurity 84% by SDS PAGE and Coomassie blue staining.
Sourceexpressed in Sf21 cells
Specific Activity1562 U/mg, where one unit of TOA2 activity is defined as 1 nmol phosphate incorporated into 0.8 mg/mL myelin basic per minute at 30°C with a final ATP concentration of 100 μM.
Entrez Gene Number
Gene Symbol
  • TAOK2
  • TAO1
  • TAO2
  • PSK
  • PSK1
  • MAP3K17
  • KIAA0881
  • hKFC-C
  • PSK-1
Protein TargetTAO2
Purification MethodNi2+/NTA-agarose
Target Sub-FamilySTE
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9UL54 # Activates the JNK MAP kinase pathway through the specific activation of the upstream MKK3 and MKK6 kinases.
COFACTOR: Magnesium (By similarity).
SIZE: 1235 amino acids; 138251 Da
SUBUNIT: Interacts with MKK3 and MKK6 (By similarity). Self- associates.
SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Multi-pass membrane protein (Potential). Note=Found to be perinuclear and localized to vesicular compartment.
TISSUE SPECIFICITY: Ubiquitously expressed, with a higher level of expression in testis and brain.
SIMILARITY: SwissProt: Q9UL54 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 protein kinase domain.
Molecular Weight138.3 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of 0.8 mg/ml myelin basic protein (MBP). Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of TAO2.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Packaging Information
Material Size10 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-736 04053252733307

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Alternative Packsize

Catalogue Number Description
14-736M TAO2 Protein, active, 250 µg

Product Families

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases