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SCC225 YUMM 1.3 Mouse Melanoma Cell Line

​ The YUMM cell models recapitulate genetic drivers found in a significant proportion of human melanomas. YUMM1.3 cells retain genetic markers of the Braf/Pten mouse models, characterized by activation of Braf and inactivation of Pten and Cdkn2a, in which melanoma tumors develop with short latency.

SCC225
>1x10^6 viable cells/vial  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCC225
DescriptionYUMM 1.3 Mouse Melanoma Cell Line
OverviewYUMM1.3 cells are syngeneic with the immunocompetent C57BL/6 mouse background and retain genetic markers of the Braf/Pten mouse models, characterized by activation of Braf and inactivation of Pten and Cdkn2a.
Alternate Names
  • Yale University Mouse Melanoma cell line
  • Yale University Mouse Melanoma 1.3 cell line
Background InformationImmunocompetent genetically-engineered mouse models harboring discrete genetic drivers of melanoma are essential for studying potential immunotherapies but are limited by the need to maintain colonies of multiple genotypes necessary to generate mouse models with appropriate genetic backgrounds.

The YUMM (Yale University Mouse Melanoma) cell lines were developed as models that recapitulate genetic drivers found in a significant proportion of human melanomas.

Source
YUMM1.3 mouse melanoma cell line was derived from a 4-hydroxytamoxifen-induced melanoma tumor in a male C57BL/6 mouse into which mutations from the Braf/Pten genetically engineered mouse model had been introduced via backcrossing. YUMM1.3 cell line harbors the Braf V600E mutation and is homozygous negative for wild-type Pten and Cdkn2.

References:
1. Meeth K et al. (2016) The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment Cell Melanoma Res 29(5): 590-597.
2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten loss to induce metastatic melanoma. Nat Genet. 41(5): 544-552.
References
Product Information
Components
  • ≥1x106 viable YUMM1.3 Cells: (Catalog No. SCC225). Store in liquid nitrogen.
Quality LevelMQ100
Applications
Application​ The YUMM cell models recapitulate genetic drivers found in a significant proportion of human melanomas. YUMM1.3 cells retain genetic markers of the Braf/Pten mouse models, characterized by activation of Braf and inactivation of Pten and Cdkn2a, in which melanoma tumors develop with short latency.
Key Applications
  • Cell Culture
Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.
Biological Information
HostMouse
Cell Line Type
  • Cancer Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
• YUMM1.3 cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Storage and Shipping Information
Storage ConditionsYUMM1.3 cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Packaging Information
Material Size>1x10^6 viable cells/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCC225 04065270305848

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