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SCM054 AOF ITS Supplement (100X), 10ml | SCM054

AOF ITS supplement is an animal-origin-free product that contains human recombinant insulin, transferrin along with sodium selenite and is used for the serum-free culture of numerous cell types.

AOF ITS Supplement (100X), 10ml | SCM054 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture, Cell Stimulation, Cell Growth, Stem Cell Culture
SCM054
10 ml  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Key Applications
CULT, Cell Stimulation, Cell Growth, Cult
Description
Catalogue NumberSCM054
DescriptionAOF ITS Supplement (100X), 10ml | SCM054
Alternate Names
  • ITS
  • Insulin Transferrin Selenite
Background InformationEMD Millipore’s AOF ITS (100X) supplement is an animal-origin-free product that contains human recombinant insulin, human recombinant transferrin along with sodium selenite.

Undefined animal derived media supplements, such as fetal bovine serum, have recently been an area of concern for many cell culturists due to their increased costs, lot to lot variability and increased regulation worldwide. The elimination of fetal bovine serum from cell culture media provides a more defined enviroment to study basic biological questions. Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies such as insulin, transferrin and selenite.

ITS supplements have been proven to replace serum and grow the following cells in serum free or reduced serum conditions: CHO, HEK293, fibroblasts, hybridoma, VERO cells, Hela, HT29, MDCK, Mesenchymal, IPS Cells, Cardiac, kidney, keratinocyte, embryonic, neural stem cell, hepatocytes.
References
Product Information
Components
  • All components are derived using AOF (Animal-Origin-Free) reagents and protocols.

    Recombinant Human Insulin is derived from Pichia pastoris (yeast).
    Recombinant Human Transferrin is derived from Oryza sativa (rice).
    Sodium Selenite is a synthetic chemical.
Control
  • VERO cells
PresentationProduct is made in an Earle's Balanced Salt Solution and filtered through a 0.22 micron membrane before vialing. Solution is a light pink clear solution that is free of particulates.
Quality LevelMQ100
Applications
ApplicationAOF ITS supplement is an animal-origin-free product that contains human recombinant insulin, transferrin along with sodium selenite and is used for the serum-free culture of numerous cell types.
Key Applications
  • Cell Culture
  • Cell Stimulation
  • Cell Growth
  • Stem Cell Culture
Application NotesEMD Millipore’s AOF ITS (100X) supplement is an completely animal-origin-free product
Biological Information
Concentration100X
Media FormLiquid
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceTested for the ability to support the proliferation of VERO cells in serum free conditions over a 3 day time period.
pH: 7.6-8.0.
Product is 0.2 μm filtered solution.
Endotoxin levels are ≤ 1 EU/mg.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at 2-8°C. Further dilutions should be made into appropriate buffer and/or medium to which protein has been added, such as DMEM/1% Human Serum Albumin or equivalent.
Packaging Information
Material Size10 ml
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
SCM054 04053252972164