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DR1086 Anti-ATF3 Mouse mAb (6B8)

Anti-ATF3, mouse monoclonal, clone 6B8, recognizes the human ATF3 protein. It is validated for ELISA and Western blotting.

Anti-ATF3 Mouse mAb (6B8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
DR1086
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

产品目录编号包装 数量 / 包装
DR1086-100UGCN 100 μg
Description
OverviewRecognizes the ATF3 protein.
Catalogue NumberDR1086
Brand Family Calbiochem®
Application Data
Detection of human ATF3 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with full-length, recombinant, human ATF3 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-ATF3 Mouse mAb (6B8) (Cat. No. DR1086). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant, human ATF3 (aa 1-182), expressed as a GST fusion protein.
Clone6B8
HostMouse
IsotypeIgG₃
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
DR1086-100UGCN 04055977226089

Documentation

Anti-ATF3 Mouse mAb (6B8) MSDS

职位

物料安全数据表 (MSDS) 

Anti-ATF3 Mouse mAb (6B8) 分析证书

标题批号
DR1086
数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-November-2010 RFH
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human ATF3 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with full-length, recombinant, human ATF3 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-ATF3 Mouse mAb (6B8) (Cat. No. DR1086). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ATF3 protein.
BackgroundActivating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (δZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.
HostMouse
ImmunogenFull-length, recombinant, human ATF3 (aa 1-182), expressed as a GST fusion protein.
Clone6B8
IsotypeIgG₃
Specieshuman
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review