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ST1027 Anti-Acetylated Lysine Rabbit pAb

Anti-Acetylated Lysine, rabbit polyclonal antibody that recognizes acetylated lysine in UV-treated HEK293 cells. It is validated for ELISA, Western blotting, immunoprecipitation, & paraffin sections.

Anti-Acetylated Lysine Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ST1027
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesRbPolyclonal Antibody

Products

产品目录编号包装 数量 / 包装
ST1027-50ULCN 塑胶安瓿;塑胶针药瓶 50 ul
Description
OverviewRecognizes acetylated lysine residues in UV-treated HEK293 cells.
Catalogue NumberST1027
Brand Family Calbiochem®
Application Data
Detection of monkey acetylated lysine by immunoblotting. Samples: Cell extracts from COS cells, untreated or TSA-treated (lanes 1 and 2) and serum starved (lanes 3 and 4). Primary antibody: Anti-Acetylated Lysine Rabbit pAb (Cat. No. ST1027) (1:1000). Detection: chemiluminescence.
References
ReferencesYu, X., et al. 2002. J. Natl. Cancer Inst. 94, 504.
Mal, A., et al. 2001. EMBO J. 20, 1739.
Sano, Y. and Ishii, S. 2001. J. Biol. Chem. 276, 3674.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Positive controlHEK293 cells treated with UV
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoprecipitation
Paraffin Sections
Application NotesELISA (1:1000)
Immunoblotting (1:1000)
Immunoprecipitation (1:100)
Paraffin Sections (1:50)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogensynthetic acetylated-lysine
HostRabbit
IsotypeIgG
Species Reactivity
  • A Broad Range Of Species
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
ST1027-50ULCN 04055977208672

Documentation

Anti-Acetylated Lysine Rabbit pAb MSDS

职位

物料安全数据表 (MSDS) 

Anti-Acetylated Lysine Rabbit pAb 分析证书

标题批号
ST1027

参考

参考信息概述
Yu, X., et al. 2002. J. Natl. Cancer Inst. 94, 504.
Mal, A., et al. 2001. EMBO J. 20, 1739.
Sano, Y. and Ishii, S. 2001. J. Biol. Chem. 276, 3674.

小册子

标题
Antibody Sourcebookﾙ Sixth Edition
数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-October-2007 RFH
ApplicationELISA (1:1000)
Immunoblotting (1:1000)
Immunoprecipitation (1:100)
Paraffin Sections (1:50)
Application Data
Detection of monkey acetylated lysine by immunoblotting. Samples: Cell extracts from COS cells, untreated or TSA-treated (lanes 1 and 2) and serum starved (lanes 3 and 4). Primary antibody: Anti-Acetylated Lysine Rabbit pAb (Cat. No. ST1027) (1:1000). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes acetylated lysine-containing proteins.
BackgroundProtein acetylation is a reversible modification that can control the activity of histones, p53, and many other proteins. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs).
HostRabbit
Immunogensynthetic acetylated-lysine
IsotypeIgG
Speciesa broad range of species
Positive controlHEK293 cells treated with UV
FormLiquid
FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
PreservativeNone
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesYu, X., et al. 2002. J. Natl. Cancer Inst. 94, 504.
Mal, A., et al. 2001. EMBO J. 20, 1739.
Sano, Y. and Ishii, S. 2001. J. Biol. Chem. 276, 3674.