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113200 Sigma-AldrichAnti-α-Actin Mouse mAb (1A4)

This Anti-α-Actin Mouse mAb (1A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of α-Actin.

Anti-α-Actin Mouse mAb (1A4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
小册子
Data Sheet

Recommended Products

概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
Bab, H, Rb	M	Monoclonal Antibody

Products

产品目录编号	包装	数量 / 包装
113200-500ULCN	塑胶安瓿;塑胶针药瓶	500 ul	添加到收藏夹添加到收藏夹添加到我的收藏夹

Description
Overview	Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial). Antibody Target Gene Symbol: ACTA2 Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN Entrez Gene Name: actin, alpha 2, smooth muscle, aorta Hu Entrez ID: 59 (Related Antibodies: 113200) Rat Entrez ID: 81633
Catalogue Number	113200
Brand Family	Calbiochem®

References
References	Rannov-Jennsen, L., et al. 1990. Lab Invest. 63, 532. Skalli, O., et al. 1989. J. Histochem. Cytochem 37, 315. Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.

Product Information
Form	Liquid
Formulation	In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.
Negative control	Fibroblasts, striated muscle, or myocardium
Positive control	Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Frozen Sections Paraffin Sections
Application Notes	Frozen Sections (1:50-1:75) Immunoblotting (see comments) Paraffin Sections (1:50-1:75)
Application Comments	Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.
Immunogen	Human Smooth Muscle
Clone	1A4
Host	Mouse
Isotype	IgG_2a
Species Reactivity	Baboon Human Rabbit
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
113200-500ULCN	04055977208054

Documentation

Anti-α-Actin Mouse mAb (1A4) MSDS

职位
物料安全数据表 (MSDS)

Anti-α-Actin Mouse mAb (1A4) 分析证书

标题	批号
113200	输入批号

参考

参考信息概述
Rannov-Jennsen, L., et al. 1990. Lab Invest. 63, 532. Skalli, O., et al. 1989. J. Histochem. Cytochem 37, 315. Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.

小册子

标题
Antibody Sourcebookﾙ Sixth Edition

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	17-July-2007 RFH
Application	Frozen Sections (1:50-1:75) Immunoblotting (see comments) Paraffin Sections (1:50-1:75)
Description	Mouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.
Background	Actin is a cytoskeletal protein. Smooth muscle actin forms smaller, less ordered contractile units than actin from skeletal or cardiac muscle. The activity and state of assembly of smooth muscle actin is controlled by calcium-regulated phosphorylation of one of the myosin light chains.
Host	Mouse
Immunogen species	Human Smooth Muscle
Immunogen	a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.
Clone	1A4
Isotype	IgG_2a
Species	baboon, human, rabbit
Positive control	Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
Negative control	Fibroblasts, striated muscle, or myocardium
Form	Liquid
Formulation	In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Rannov-Jennsen, L., et al. 1990. Lab Invest. 63, 532. Skalli, O., et al. 1989. J. Histochem. Cytochem 37, 315. Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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