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NB20 Anti-AnkyrinG Mouse mAb (463)

Anti-Ankyrin G, mouse monoclonal, clone 463, recognizes the ~190 kDa ankyrin G protein in brain extracts. It is validated for Western blotting, immunofluorescence, and frozen sections.

Anti-AnkyrinG Mouse mAb (463) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
NB20
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概述

Replacement Information

重要规格表

Species ReactivityHostAntibody Type
Ca, H, M, RMMonoclonal Antibody

Products

产品目录编号包装 数量 / 包装
NB20-100UGCN 塑胶安瓿;塑胶针药瓶 100 μg
Description
OverviewRecognizes the ~190 kDa ankyrinG protein in brain extracts. Reacts with all spliced forms of ankyrinG.
  • Antibody Target Gene Symbol: ANK3
  • Target Synonym: 2900054D09RIK, AI314020, AnkG, Ankyrin-3, ANKYRIN-G, FLJ45464, MGC14049
  • Entrez Gene Name: ankyrin 3, node of Ranvier (ankyrin G)
  • Hu Entrez ID: 288 (Related Antibodies: AP1034, PC523, PC522, AP1057)
  • Mu Entrez ID: 11735
  • Rat Entrez ID: 361833
    		•
    Catalogue NumberNB20
    Brand Family Calbiochem®
    Application Data
    Detection of mouse AnkyrinG by immunoblotting. Sample: Whole tissue extract from mouse brain. Primary antibody: Anti-AnkyrinG Mouse mAb (463) (Cat. No. NB20) (2 µg/ml) Detection: chemiluminescence.
    References
    ReferencesDavis, J.Q., et al. 1995. Mol. Biol. Cell 6, 177a, supplement.
    Kunimoto, M., et al. 1995. FEBS Lett. 357, 217.
    Lambert, S., et al. 1995. Mol. Biol. Cell 6, 98a supplement.
    Bennett, V. and Gilligan, D.M. 1993. Ann. Rev. Cell Biol. 9, 27.
    Kunimoto, M., et al. 1991. J. Cell Biol. 115, 1319.
    Otto, E., et al. 1991. J. Cell Biol. 114, 241.
    Davis, J., and Bennett, V. 1984. J. Biol. Chem. 259, 13550.
    Product Information
    FormLiquid
    FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
    Positive controlMouse or rat brain extract or unmyelinated axons
    Preservative≤0.1% sodium azide
    Quality LevelMQ100
    Applications
    Key Applications Frozen Sections
    Immunoblotting (Western Blotting)
    Immunofluorescence
    Application NotesFrozen Sections (1-2 µg/ml)
    Immunoblotting (2-5 µg/ml)
    Immunofluorescence (1-2 µg/ml)
    Application CommentsAnkyrinG is a good immunohistochemical marker for axon initial segments, nodes of Ranvier, and epithelial cell-cell contact sites. Antibody should be titrated for optimal results in individual systems.
    Biological Information
    Immunogena synthetic peptide corresponding to amino acids from the spectrin-binding domain of human ankyrinG
    ImmunogenHuman
    Clone463
    HostMouse
    Purity≥95% by SDS-PAGE
    IsotypeIgG₁
    Species Reactivity
    • Canine
    • Human
    • Mouse
    • Rat
    Antibody TypeMonoclonal Antibody
    Concentration Label Please refer to vial label for lot-specific concentration
    Physicochemical Information
    Dimensions
    Materials Information
    Toxicological Information
    Safety Information according to GHS
    Safety Information
    Product Usage Statements
    Storage and Shipping Information
    Ship Code Blue Ice Only
    Toxicity Standard Handling
    Storage +2°C to +8°C
    Do not freeze Yes
    Packaging Information
    Transport Information
    Supplemental Information
    Specifications
    Global Trade Item Number
    产品目录编号 GTIN
    NB20-100UGCN 04055977209808

    Documentation

    Anti-AnkyrinG Mouse mAb (463) MSDS

    职位

    物料安全数据表 (MSDS) 

    Anti-AnkyrinG Mouse mAb (463) 分析证书

    标题批号
    NB20

    参考

    参考信息概述
    Davis, J.Q., et al. 1995. Mol. Biol. Cell 6, 177a, supplement.
    Kunimoto, M., et al. 1995. FEBS Lett. 357, 217.
    Lambert, S., et al. 1995. Mol. Biol. Cell 6, 98a supplement.
    Bennett, V. and Gilligan, D.M. 1993. Ann. Rev. Cell Biol. 9, 27.
    Kunimoto, M., et al. 1991. J. Cell Biol. 115, 1319.
    Otto, E., et al. 1991. J. Cell Biol. 114, 241.
    Davis, J., and Bennett, V. 1984. J. Biol. Chem. 259, 13550.
    数据表

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision05-September-2007 RFH
    ApplicationFrozen Sections (1-2 µg/ml)
    Immunoblotting (2-5 µg/ml)
    Immunofluorescence (1-2 µg/ml)
    Application Data
    Detection of mouse AnkyrinG by immunoblotting. Sample: Whole tissue extract from mouse brain. Primary antibody: Anti-AnkyrinG Mouse mAb (463) (Cat. No. NB20) (2 µg/ml) Detection: chemiluminescence.
    DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63/Ag 8.653 mouse myeloma cells. Recognizes the ~190 kDa isoform of AnkyrinG.
    BackgroundAnkyrins are a family of spectrin-binding proteins that couple the spectrin/actin cytoskeletal network to the cytoplasmic domains of integral membrane proteins which include channels such as the anion exchanger, voltage-dependent sodium channel, and the Na/K ATPase, as well as neuronal cell adhesion molecules related to L1. In the nervous system, three different ankyrins are known to exist; ankyrinB, ankyrinG, and ankyrinR. All three ankyrins contain an N-terminal membrane binding domain, an internal domain capable of binding spectrin, and a C-terminal regulatory domain which is the target of alternative splicing. AnkyrinB, the predominant neuronal ankyrin includes two isoforms of ~220 kDa and ~440 kDa. The ~220 kDa alternatively spliced isoform is the major form present in the adult brain, while the ~440 kDa form is preferentially expressed in the developing neonatal brain. Three forms of ankyrinG (~190 kDa, ~270 kDa, ~480 kDa) are localized in axonal initial segments and the nodes of Ranvier of myelinated axons where fluxes of action potential occur and ion channels are enriched. Ankyrins comprise ~0.5%-1% of the total membrane protein in the adult vertebrate brain.
    HostMouse
    Immunogen speciesHuman
    Immunogena synthetic peptide corresponding to amino acids from the spectrin-binding domain of human ankyrinG
    Clone463
    IsotypeIgG₁
    Speciescanine, human, mouse, rat
    Positive controlMouse or rat brain extract or unmyelinated axons
    FormLiquid
    FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
    Concentration Label Please refer to vial label for lot-specific concentration
    Purity≥95% by SDS-PAGE
    Preservative≤0.1% sodium azide
    CommentsAnkyrinG is a good immunohistochemical marker for axon initial segments, nodes of Ranvier, and epithelial cell-cell contact sites. Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    ReferencesDavis, J.Q., et al. 1995. Mol. Biol. Cell 6, 177a, supplement.
    Kunimoto, M., et al. 1995. FEBS Lett. 357, 217.
    Lambert, S., et al. 1995. Mol. Biol. Cell 6, 98a supplement.
    Bennett, V. and Gilligan, D.M. 1993. Ann. Rev. Cell Biol. 9, 27.
    Kunimoto, M., et al. 1991. J. Cell Biol. 115, 1319.
    Otto, E., et al. 1991. J. Cell Biol. 114, 241.
    Davis, J., and Bennett, V. 1984. J. Biol. Chem. 259, 13550.