Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABN1356 Anti-C9ORF72/C9RANT (poly-PA)

Anti-C9ORF72/C9RANT (poly-PA), Cat. No. ABN1356, is a highly specific rabbit polyclonal antibody that targets C9ORF72(poly-PA) and has been tested in Dot Blot, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Anti-C9ORF72/C9RANT (poly-PA) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: C9orf72 UniProt Number: Q96LT7
ABN1356
100 µL  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, DB, IF, IHCRbSerumPolyclonal Antibody
Description
Catalogue NumberABN1356
DescriptionAnti-C9ORF72/C9RANT (poly-PA)
Alternate Names
  • C9ORF72/C9RANT (poly-PA)
  • alpha-PA-antisense
Background InformationExpansion of a GGGGCC (G4C2) hexanucleotide repeat sequence in the non-coding region of human chromosome 9 open reading frame 72 or C9orf72 (also known as ALSFTD, FTDALS; Gene ID 203228) is the most common genetic abnormality in familial and sporadic frontotemporal dementia (FTD) and motor neuron disease (MND), with amyotrophic lateral sclerosis (ALS) as the most frequent form. The number of hexanucleotide repeats in the normal population ranges from 2 to 24, whereas up to several thousand repeats (700 - 4,400 repeats) are found in the pathologically expanded allele. Unconventional repeat-associated non-ATG-initiated translation (RANT) of the GGGGCC repeats in the three alternate reading frames from both sense and antisense transcripts generates five types of dipeptide repeat (DPR) protein species, each composed of repeating units of two amino acids, glycine-alanine (GA), glycine-proline (GP), glycine-arginine (GR), alanine-proline (AP), proline-arginine (PR), respectively. The sense transcript (5'-GGGGCC-3' hexanucleotide repeats) is translated through repeat-associated non-ATG (RAN) translation such that poly- (Gly- Ala), poly-(Gly-Pro), and poly-(Gly-Arg) proteins were produced. The anti-sense transcript (containing 5'-GGCCCC-3' (G2C4) hexanucleotide repeats is translated through repeat-associated non-ATG (RAN) translation producing poly-(Pro-Ala), poly- (Pro-Arg), poly- (Gly-Pro) proteins. The antisense proteins accumulate in cytoplasmic aggregates in affected brain regions, including the frontal and motor cortex, hippocampus, and spinal cord neurons.
References
Product Information
FormatSerum
PresentationRabbit polyclonal serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-C9ORF72/C9RANT (poly-PA), Cat. No. ABN1356, is a highly specific rabbit polyclonal antibody that targets C9ORF72(poly-PA) and has been tested in Dot Blot, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Key Applications
  • Western Blotting
  • Dot Blot
  • Immunofluorescence
  • Immunohistochemistry
Application NotesImmunofluorescence Analysis: A representative lot detected C9ORF72/C9RANT (poly-PA) in transfected HEK293T Flag-PA cells (Zu, T., et. al. (2013). Proc. Natl. Acad. Sci. USA. 110(51):E4968-77).

Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected C9ORF72/C9RANT (poly-PA) in Flag-PA trannsfected HEK293T cells (Courtesy of Dr. Laura P.W. Ranum and Dr. Lien Nguyen from the University of Florida).


Western Blotting Analysis: A representative lot detected C9ORF72/C9RANT (poly-PA) in recombinant Protein (Zu, T., et. al. (2013). Proc. Natl. Acad. Sci. USA. 110(51):E4968-77).

Immunohistochemistry Analysis: A representative lot detected C9ORF72/C9RANT (poly-PA) in aggregates found in neurons of the CA and DG regions of the hippocampus (Zu, T., et. al. (2013). Proc. Natl. Acad. Sci. USA. 110(51):E4968-77).

Dot Blotting Analysis: A representative lot detected C9ORF72/C9RANT (PR-CT) in Dot Blotting applications (Zu, T., et. al. (2013). Proc Natl Acad Sci USA. 110(51):E4968-77).
Biological Information
ImmunogenH2N-APAPAPAPAPAPAPACKKKK-amide.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody recognizes C9ORF72/C9RANT (poly-PA).
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • C9orf72
Purification MethodUnpurified
UniProt Number
Molecular Weight~35 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from HEK293 cells transfected with Flag-PA.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected C9ORF72/C9RANT (poly-PA) in 10 µL of lysate from HEK293 cells transfected with Flag-PA.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
ABN1356 04054839167454