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ABT66 Anti-CADM1 Antibody

Detect CADM1 using this Anti-CADM1 Antibody validated for use in WB & IC.

Anti-CADM1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: CADM1, IGSF4, IGSF4A, NECL2, SYNCAM, TSLC1 UniProt Number: Q9BY67
ABT66
100 µg  
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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABT66
DescriptionAnti-CADM1 Antibody
Alternate Names
  • Cell adhesion molecule 1
  • Immunoglobulin superfamily member 4
  • IgSF4
  • Nectin-like protein 2
  • NECL-2
  • Spermatogenic immunoglobulin superfamily
  • SgIgSF
  • Synaptic cell adhesion molecule
  • SynCAM
  • Tumor suppressor in lung cancer 1
  • TSLC-1
Background InformationCADM1 (also known as cell adhesion molecule 1, Nectin-like protein 2, synaptic cell adhesion molecule. IgSF4 and TSLC-1) is a tumor suppressor part of the immunoglobulin superfamily and has been found to show reduced expression in many different solid tumors. CADM1 has also been found to play a role in the formation of epithelia-like cell structure. Recent research has also linked CADM1 to mediating emotional and social behaviors and motor skills. CADM1 may also be important in psychiatric and behavioral disorders such as autism.
References
Product Information
FormatAffinity Purified
Control
  • PC3 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect CADM1 using this Anti-CADM1 Antibody validated for use in WB & IC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected CADM1 in NIH/3T3, A431, and HeLa cells.
Biological Information
ImmunogenLinear peptide corresponding to human CADM1.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CADM1
  • IGSF4
  • IGSF4A
  • NECL2
  • SYNCAM
  • TSLC1
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Mediates homophilic cell-cell adhesion in a Ca2+-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca2+-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. May act as a synaptic cell adhesion molecule that drives synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.

SUBUNICT STRUCTURE: Homodimer. Interacts with CRTAM and EPB41L3/DAL1. The interaction with EPB41L3/DAL1 may act to anchor CADM1 to the actin cytoskeleton. Interacts via its C-terminus with the PDZ domain of MPP3 and the PDZ domain of MPP6.

SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Note: Associates with perinuclear and plasma membranes in vivo. Localized to the basolateral plasma membrane of epithelial cells in gall bladder. Ref.4 UniProtKB Q8R5M8

DOMAIN: The cytoplasmic domain appears to play a critical role in proapoptosis and tumor suppressor activity in NSCLC. Ref.7 Ref.11

SEQUENCE SIMILARITIES: Belongs to the nectin family.

Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Molecular Weight~48 kDa & 37 kDa observed.
Isoform 1 (48 kDa), Isoform 2 (37 kDa).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in PC3 cell lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected CADM1 in 10 µg of PC3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
ABT66 04053252420641