Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABN2254 Anti-Catenin Delta-2 Antibody, clone 12B4

Anti-Catenin Delta-2 Antibody, clone 12B4, Cat. No. MABN2254, is a highly specific mouse monoclonal antibody that targets CTNND2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Catenin Delta-2 Antibody, clone 12B4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: CTNND2, NPRAP UniProt Number: Q9UQB3
MABN2254
100 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN2254
DescriptionAnti-Catenin Delta-2 Antibody, clone 12B4
Alternate Names
  • Catenin delta-2
  • GT24
  • Neural plakophilin-related ARM-repeat protein
  • NPRAP
  • Neurojungin
Background InformationCatenin delta-2 (UniProt: Q9UQB3; also known as Delta-catenin, GT24, Neural plakophilin-related ARM-repeat protein, NPRAP, Neurojungin) is encoded by the CTNND2 (also known as NPRAP) gene (Gene ID: 1501) in human. Delta Catenin is a component of the cadherin-catenin cell adhesion complex and is enriched in synaptosomes. Catenin Delta 2 is a member of the beta-catenin superfamily and is expressed in the brain where it is required for normal cognitive development and its highest expression is observed in fetal brain. Catenin Delta 2 is phosphorylated by Cdk5 and by GSK-3beta. Catenin Delta 2 plays a critical role in neuronal development, particularly in the formation and/or maintenance of dendritic spines and synapses. It is involved in the regulation of Wnt signaling where it acts on beta-catenin turnover, facilitating beta-catenin interaction with GSK3beta, phosphorylation, ubiquitination, and degradation. When bound to ZBTB33 (Zinc Finger and BTB Domain Containing 33), it functions as a transcriptional activator. The loss of CTNND2 gene has been associated with the intellectual disability observed in the cri du chat syndrome that is characterized by slow growth, microcephaly, delayed acquisition of psychomotor skills and intellectual disability. (Ref.: Yuan, L et al. (2015). J. Biol. Chem. 290(17):10947-57).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Catenin Delta-2 Antibody, clone 12B4, Cat. No. MABN2254, is a highly specific mouse monoclonal antibody that targets CTNND2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected Catenin Delta-2 in human cerebellum, human cerebral cortex, rat hippocampus, and rat cerebral cortex tissues.
Biological Information
ImmunogenGST/His-tagged recombinat fragment corresponding to 104 amino acids from the C-terminal half of human CTNND2.
Clone12B4.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 12B4.1 specifically detects CTNND2 in rat brain microsomal preparations. This clone targets a sequeence within 104 amino acids in the C-terminal half of CTNND2.
IsotypeIgG2bκ
Species Reactivity
  • Rat
  • Human
Species Reactivity NoteHuman, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CTNND2
  • NPRAP
Purification MethodProtein G purified
UniProt Number
Molecular Weight~136 kDa observed; 132.66 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat brain microsomal prep tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Catenin Delta-2 in 10 µg of rat brain microsomal preparations.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABN2254 04054839168253