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IM03 Anti-Cathepsin D (Ab-1) Mouse mAb (BC011)

Anti-Cathepsin D (Ab-1), mouse monoclonal, clone BC011, recognizes latent and mature form and the ~34 kDa subunit in breast carcinoma tissue extracts. It is validated for WB and for paraffin sections.

Anti-Cathepsin D (Ab-1) Mouse mAb (BC011) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
IM03
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概述

Replacement Information

重要规格表

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

产品目录编号包装 数量 / 包装
IM03-100UGCN 塑胶安瓿;塑胶针药瓶 100 μg
Description
OverviewRecognizes the ~52 kDa latent form, the ~48 kDa mature form, and the ~34 kDa subunit of cathepsin D in breast carcinoma tissue extracts.
Catalogue NumberIM03
Brand Family Calbiochem®
References
ReferencesRochefort, H. 1992. Eur. J. Cancer. 28A, 1780.
Rochefort, H. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 153.
Westly, B. and Rochefort, H. 1980. Cell 20, 352.
Barrett, A.J. 1977. in Proteinases in Mammalian Cells and Tissues, ed. Barret, A.J. (North Holland, New York), pp. 209.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Positive controlSK-HEP-1 concentrated conditioned medium or breast carcinoma tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesImmunoblotting (1 µg/ml)
Paraffin Sections (5 µg/ml, trypsin pre-treatment required)
Application CommentsDoes not detect the ~14 kDa subunit of cathespin D, nor will it detect cathespins B or L. Cathepsins are secreted and accumulate in conditioned tissue culture media and can be detected by immunoblotting from this source. Some cell extracts do not contain sufficient cathepsin for detection by immunoblotting. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenmature, human cathepsin D
ImmunogenHuman
CloneBC011
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
IM03-100UGCN 04055977228137

Documentation

Anti-Cathepsin D (Ab-1) Mouse mAb (BC011) MSDS

职位

物料安全数据表 (MSDS) 

Anti-Cathepsin D (Ab-1) Mouse mAb (BC011) 分析证书

标题批号
IM03

参考

参考信息概述
Rochefort, H. 1992. Eur. J. Cancer. 28A, 1780.
Rochefort, H. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 153.
Westly, B. and Rochefort, H. 1980. Cell 20, 352.
Barrett, A.J. 1977. in Proteinases in Mammalian Cells and Tissues, ed. Barret, A.J. (North Holland, New York), pp. 209.

小册子

标题
Cathepsins and Related Products Technical Bulletin
数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-August-2007 RFH
ApplicationImmunoblotting (1 µg/ml)
Paraffin Sections (5 µg/ml, trypsin pre-treatment required)
DescriptionPurified mouse monoclonal antibody generated by immunizing CB6F1 mice with the specified immunogen and fusing splenocytes with SP2/0 cells. Recognizes the ~52 kDa pro-cathespin D, the ~48 kDa mature cathepsin D, and the ~34 kDa subunit of cathespin D.
BackgroundCathepsin D is a normal lysosomal proteinase which is expressed in all cells. It is an aspartyl proteinase with a pH optimum in the range of 3-5 and contains two N-linked oligosaccharides. Cathepsin D is synthesized as an inactive 52 kDa pro-enzyme. Activation involves the proteolytic removal of the 43 amino acid pro-fragment and internal cleavage to generate the two chain form made up of 34 and 14 kDa subunits. Cathepsin D contains the mannose-6-phosphate lysosomal localization signal which targets the enzyme to the lysosomal compartment where it functions in the normal degradation of proteins. In certain tumor cells, cathepsin D is abnormally processed and is secreted in its 52 kDa precursor form. Numerous clinical studies as well as in vitro evidence suggest that cathepsin D plays an important role in malignant transformation and may be a useful prognostic indicator for breast cancer.
HostMouse
Immunogen speciesHuman
Immunogenmature, human cathepsin D
CloneBC011
IsotypeIgG2a
Specieshuman
Positive controlSK-HEP-1 concentrated conditioned medium or breast carcinoma tissue
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsDoes not detect the ~14 kDa subunit of cathespin D, nor will it detect cathespins B or L. Cathepsins are secreted and accumulate in conditioned tissue culture media and can be detected by immunoblotting from this source. Some cell extracts do not contain sufficient cathepsin for detection by immunoblotting. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesRochefort, H. 1992. Eur. J. Cancer. 28A, 1780.
Rochefort, H. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 153.
Westly, B. and Rochefort, H. 1980. Cell 20, 352.
Barrett, A.J. 1977. in Proteinases in Mammalian Cells and Tissues, ed. Barret, A.J. (North Holland, New York), pp. 209.