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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

17-347B Sigma-AldrichAnti-Citrulline (Modified) Detection Kit

The Anti-Citrulline (Modified) Detection Kit provides reagents for the modification and detection of citrulline-containing proteins by Western blot (immublot) analysis.

Anti-Citrulline (Modified) Detection Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology

Recommended Products

概述

Replacement Information

重要规格表

Species Reactivity	Key Applications	Host
A	WB	Human Recombinant

Description
Catalogue Number	17-347B
Replaces	17-347
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-Citrulline (Modified) Detection Kit
Overview	The Anti-Citrulline (Modified) Detection Kit provides reagents for the modification and detection of citrulline-containing proteins by Western blot analysis. Modification of citrulline residues is created by a chemical reaction with 2,3-butanedione monoxime and antipyrine in a strong acid solution. Detection of the modified citrulline proteins uses a standard immunoblot protocol with an anti-Citrulline (modified) Human monoclonal antibody and a goat anti-Human IgG secondary antibody horseradish peroxidase (HRP) conjugate. This type of citrulline modification ensures the detection of citrulline residues in proteins regardless of neighboring amino acid sequences.
Materials Required but Not Delivered	1. 98% H2SO4 (Sulfuric Acid) 2. 85% H3PO4 (Phosphoric Acid) 3. TBS-TWEEN® Tablets (EMD Millipore catalog #524753) 4. Luminata Crescendo Western HRP substrate (EMD Millipore catalog #WBLUR100) 5. Non-fat dry milk (EMD Millipore Catalog # 20-200)
Background Information	Citrullination or deimination is the post-translational modification of arginine residues to citrulline residues in proteins. This process is catalyzed by enzymes called peptidylarginine deiminases (PADs).

References

Product Information
Components	Anti-Modified Citrulline, clone C4 (Part number MABS487). One vial containing 100 µL of Protein A purified recombinant human monoclonal IgG1λ in PBS containing 0.05% sodium azide. Goat Anti-Human IgG, HRP Conjugate: (Part number CS216591). One vial containing 50 µL of Goat anti-Human IgG conjugated to horseradish peroxidase (HRP) in PBS, pH 7.6, with 7.5 mg/mL BSA and diluted with Stabilzyme® and glycerol to 50%. Reagent B: (Part number 20-253). One bottle containing 50 mL of 0.5% 2,3-butanedione monoxime, 0.25% antipyrine, 0.5 M acetic acid. 0.5% FeCl3: (Part number 20-255). One bottle containing 4 mL of 0.5% FeCl3. Frozen solution.
Quality Level	MQ300

Product Information

Components

Anti-Modified Citrulline, clone C4 (Part number MABS487). One vial containing 100 µL of Protein A purified recombinant human monoclonal IgG1λ in PBS containing 0.05% sodium azide.
Goat Anti-Human IgG, HRP Conjugate: (Part number CS216591). One vial containing 50 µL of Goat anti-Human IgG conjugated to horseradish peroxidase (HRP) in PBS, pH 7.6, with 7.5 mg/mL BSA and diluted with Stabilzyme® and glycerol to 50%.
Reagent B: (Part number 20-253). One bottle containing 50 mL of 0.5% 2,3-butanedione monoxime, 0.25% antipyrine, 0.5 M acetic acid.
0.5% FeCl3: (Part number 20-255). One bottle containing 4 mL of 0.5% FeCl3. Frozen solution.

Quality Level

MQ300

Applications
Application	The Anti-Citrulline (Modified) Detection Kit provides reagents for the modification and detection of citrulline-containing proteins by Western blot (immublot) analysis.
Key Applications	Western Blotting

Biological Information
Host	Human Recombinant
Species Reactivity	All

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store Anti-Modified Citrulline monoclonal antibody at 2-8°C. Store Goat Anti-Human IgG, HRP Conjugate, Reagent B, and 0.5% FeCl3 at -20°C for 4 months from date of receipt.

Packaging Information
Material Size	1 kit
Material Package	Kit capacity: 10 western blots

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
17-347B	04055977174137

Documentation

Anti-Citrulline (Modified) Detection Kit MSDS

职位
物料安全数据表 (MSDS)

Anti-Citrulline (Modified) Detection Kit 分析证书

标题	批号
Anti-Citrulline (Modified) Detection Kit - 3017763	3017763
Anti-Citrulline (Modified) Detection Kit - 3159042	3159042
Anti-Citrulline (Modified) Detection Kit - 3166769	3166769
Anti-Citrulline (Modified) Detection Kit - 3183436	3183436
Anti-Citrulline (Modified) Detection Kit - 3206073	3206073
Anti-Citrulline (Modified) Detection Kit - 3229599	3229599
Anti-Citrulline (Modified) Detection Kit - 3263094	3263094
Anti-Citrulline (Modified) Detection Kit - 3314359	3314359
Anti-Citrulline (Modified) Detection Kit - 3393749	3393749
Anti-Citrulline (Modified) Detection Kit - 3478349	3478349

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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