Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABE2600-25UL Anti-DNA Helicase B (HELB)

Anti-DNA Helicase B (HELB) Antibody, Cat. No. ABE2600, is a highly specific rabbit polyclonal antibody that targets DNA helicase B (HELB) and has been tested for use in Immunocytochemistry and Western Blotting.

Anti-DNA Helicase B (HELB) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Helb UniProt Number: Q6NVF4
ABE2600-25UL
25 µL  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
MICC, WBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABE2600-25UL
DescriptionAnti-DNA Helicase B (HELB)
Alternate Names
  • HELB
Background InformationDNA helicase B (UniProt: Q6NVF4; also known as EC: 3.6.4.12, HELB) is encoded by the Helb gene (Gene ID: 117599) in murine species. HELB helicase B is is a predominantly nuclear helicase that can undergo phosphorylation at Serine 942 by CDK2 during the G1/S transition that results in its export into the cytoplasm as cells approach and progress through S phase. Its nuclear export signal lies within amino acids 1022-1046. It serves as a 5 -3' DNA helicase involved in DNA damage response by acting as an inhibitor of DNA end resection. It is shown to bind to RPA1 and this interaction promotes HELB recruitment to chromatin following DNA damage. Following DNA damage it is recruited to sites of double-strand breaks by the Replication Protein A (RPA) complex. Recruitment to single-stranded DNA (ssDNA) following DNA damage leads to inhibit the nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5'-3' ssDNA translocase activity of HELB. HELB is reported to act independently of TP53BP1. Loss of DNA helicase B results in PARP inhibitor resistance in BRCA1-deficient tumor cells.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH7.4 with 0.05% sodium azide.
Applications
ApplicationAnti-DNA Helicase B (HELB) Antibody, Cat. No. ABE2600, is a highly specific rabbit polyclonal antibody that targets DNA helicase B (HELB) and has been tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application NotesImmunocytochemistry Analysis: A representative lot detected DNA Helicase B (HELB) in Immunocytochemistry applications (Tkac, J., et. al. (2016). Mol Cell. 61(3):405-418).

Western Blotting Analysis: A representative lot detected DNA Helicase B (HELB) in Western Blotting applications (Tkac, J., et. al. (2016). Mol Cell. 61(3):405-418).
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 227 amino acids from the C-terminal half of murine DNA helicase B.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects DNA helicase B in murine species. It targets an epitope within 227 amino acids from the C-terminal region.
IsotypeIgG
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Helb
Purification MethodAffinity Purified
UniProt Number
Molecular Weight145 kDa observed; 121.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in wild-type mouse embryonic fibroblasts .

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DNA helicase B (HELB) in lysate from wild-type mouse embryonic fibroblasts (MEF) and MEF heterogygotes for HELB.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
ABE2600-25UL 04054839468889