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05-593-I-25UL Anti-α-Dystroglycan Antibody, clone IIH6C4

Anti-a-Dystroglycan, clone IIH6C4, Cat. No. 05-593-I, is a mouse monoclonal antibody that detects Dystroglycan and is tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence Inhibition, Immunohistochemistry, and Western Blotting.

05-593-I-25UL
25 µL  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number05-593-I-25UL
DescriptionAnti-α-Dystroglycan Antibody, clone IIH6C4
Alternate Names
  • Dystroglycan (UniProt: Q28685
  • also known as Dystrophin-associated glycoprotein 1)
Background InformationDystroglycan (UniProt: Q28685; also known as Dystrophin-associated glycoprotein 1) is encoded by the DAG1 gene (Gene ID: 100009278) in rabbit. Dystroglycan is a component of the dystrophin glycoprotein complex that serves as an adhesion molecule and links the extracellular matrix to the intracellular actin cytoskeleton. Its expression is reported in the brain, cardiac muscle, skeletal muscle, and placenta. It is synthesized with a signal peptide (aa 1-29) that is subsequently cleaved off to generate the mature protein that is further autolytically cleaved (between 653-654) to produce a-dystroglycan (aa 30-653) and b-dystroglycan (aa 654-895). a-dystroglycan is an extracellular peripheral glycoprotein that binds to several extracellular matrix and synaptic proteins such as laminin-2 and 5, agrin, neurexin, and pikachurin. It is heavily O-glycosylated, which comprises of up to two thirds of its mass and the carbohydrate composition differs depending on tissue type. It is reported that its O-mannosyl glycosylation is required for its ligand- binding functions. b-dystroglycan is a single-pass type 1 transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton and serves as a cell adhesion receptor in both muscle and non-muscle tissues. In the cell, b-dystroglycan binds to dystrophin that is linked to actin cytoskeleton. Hence, a/b dystroglycans act as a molecular axis connecting extracellular matrix with the cytoskeleton across the plasma membrane. Defects in O-mannosyl glycan have been linked to various congenital muscular dystrophies caused by aberrant a-dystroglycan glycosylation. (Ref.: Kanagawa, M., et al. (2010). J. Biol. Chem. 285(41); 31208-31216; Yamada, H., et al. (1994). FEBS Lett. 352(1); 49-53; Ervasti, JM., and Campbell, KP. (1993). J. Cell Biol. 12(4); 809-823; Ibraghimov-Beskrovnaya, O., et al. (1992). Nature. 355(6362); 696-702).
References
Product Information
FormatUnpurified
PresentationMouse monoclonal antibody without azide.
Quality LevelMQ200
Applications
ApplicationAnti-a-Dystroglycan, clone IIH6C4, Cat. No. 05-593-I, is a mouse monoclonal antibody that detects Dystroglycan and is tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence Inhibition, Immunohistochemistry, and Western Blotting.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunofluorescence
  • Inhibition
  • Immunohistochemistry
  • Western Blotting
Application NotesTested Applications

Western Blotting Analysis: A representative lot detected Dystroglycan in Western Blotting applications (Azzag, K., et. al. (2020). Skelet Muscle. 10(1):10 ; Bijata, M., et. al. (2015). Front Cell Neurosci. 9:199).

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Dystroglycan in mouse heart and mouse skeletal muscle tissue sections.

Flow Cytometry Analysis: A representative lot detected Dystroglycan in Flow Cytometry applications (Gerin, I., et. al. (2016). Nat Commun. 7:11534).

Immunofluorescence Analysis: A representative lot detected Dystroglycan in Immunofluorescence applications (Azzag, K., et. al. (2020). Skelet Muscle. 10(1):10).

Inhibition Analysis: A representative lot reduced agrin-induced ERK activation in cardiac cells. (Bassat, E., et. al. (2017). Nature. 547(7662):179-184)

Immunohistochemistry Applications: A representative lot detected -Dystroglycan in Immunohistochemistry applications (Cataldi, M.P., et. al. (2018). Nat Commun. 9(1):3448).

Immunocytochemistry Analysis: A representative lot detected Dystroglycan in Immunocytochemistry applications (Bijata, M., et. al. (2015). Front Cell Neurosci. 9:199).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenRabbit skeletal muscle membrane preparation.
EpitopeUnknown
CloneIIH6 (or IIH6C4)
ConcentrationPlease refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone IIH6 (IIH6C4) is a mouse monoclonal antibody that detects Dystroglycan (a-Dystroglycan and uncleaved form) in multiple species.
IsotypeIgM
Species Reactivity
  • Mouse
  • Human
  • Rabbit
Species Reactivity NoteHuman, Mouse, Rabbit,. Predicted to react with Canine, Guinea Pig, Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • DAG1
Purification MethodUnpurified
UniProt Number
Molecular Weight~43 and ~ 156 kDa observed for a-Dystroglycan and uncleaved form ; 97.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Rabbit skeletal muscle tissue lysate.

Western Blotting Analysis: 1:1,000 of this antibody detected Dystroglycan in Rabbit skeletal muscle tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
05-593-I-25UL 04065266298314

Documentation

Anti-α-Dystroglycan Antibody, clone IIH6C4 MSDS

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物料安全数据表 (MSDS) 

Anti-α-Dystroglycan Antibody, clone IIH6C4 分析证书

标题批号
Anti-α-Dystroglycan, clone IIH6C4 - 3863493 3863493
Anti-α-Dystroglycan, clone IIH6C4 - 3891879 3891879
Anti-α-Dystroglycan, clone IIH6C4 - 3928127 3928127
Anti-α-Dystroglycan, clone IIH6C4 - 3928806 3928806
Anti-α-Dystroglycan, clone IIH6C4 - 3952765 3952765
Anti-α-Dystroglycan, clone IIH6C4 - 3984676 3984676
Anti-α-Dystroglycan, clone IIH6C4 - 3985681 3985681
Anti-α-Dystroglycan, clone IIH6C4 - 4001608 4001608
Anti-α-Dystroglycan, clone IIH6C4 - 4005758 4005758
Anti-α-Dystroglycan, clone IIH6C4 - 4012165 4012165
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