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ABT63 Anti-Ena/VASP-like protein Antibody

Detect Ena/VASP-like protein using this Anti-Ena/VASP-like protein Antibody validated for use in WB & IC.

Anti-Ena/VASP-like protein Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: EVL, RNB6 UniProt Number: Q9UI08
ABT63
100 µg  
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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, ChpWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABT63
DescriptionAnti-Ena/VASP-like protein Antibody
Alternate Names
  • Ena/VASP-like protein
  • Ena/vasodilator-stimulated phosphoprotein-like
Background InformationEna (VASP-like protein) is involved in many different cellular functions including cell migration and axon guidance. Studies have shown that Ena expression is significantly increased in breast cancer and that the level of expression is associated with tumor stage. Ena has also been linked to inner ear and hair cell development.
References
Product Information
FormatAffinity Purified
Control
  • Ramos cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Ena/VASP-like protein using this Anti-Ena/VASP-like protein Antibody validated for use in WB & IC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Ena/VASP-like protein in NIH/3T3 cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the EVH2 region of human Ena/VASP-like protein.
EpitopeEVH2 region
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes Ena/VASP-like protein at the EVH2 region.
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Chimpanzee
Species Reactivity NoteDemonstrated to react with Human and Mouse.
Predicted to react with Rat and Chimpanzee based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • EVL
  • RNB6
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization. Required to transform actin polymerization into active movement for the propulsive force of Listeria monocytogenes (By similarity).

SUBUNIT STRUCTURE: Homotetramer (By similarity). Binds to the SH3 domains of ABL1, LYN and SRC. Also binds to profilin, with preference for isoform IIa of PFN2, and the WW domain of APBB1/FE65. Binds to SEMA6A. Interacts, via the Pro-rich region, with the C-terminal SH3 domain of DNMBP. Interacts with RAPH1. Binds, via the EVH1 domain, the Pro-rich domain of Listeria monocytogenes actA (By similarity). Binds, via the EVH1 domain, the Pro-rich domain of ZYX.

SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton (By similarity). Cell projection › lamellipodium (By similarity). Note: Targeted to the leading edge of lamellipodia and the dital tip of stress fibers through interaction with a number of proteins. In activated T-cells, localizes to the F-actin collar and the distal tip of microspikes (By similarity).

DOMAIN: The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.

PTM: Phosphorylated by PKA; phosphorylation abolishes binding to SH3 domains of ABL and SRC (By similarity).

SEQUENCE SIMILARITIES: Belongs to the Ena/VASP family.

Contains 1 WH1 domain.
Molecular Weight~46 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in Ramos cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Ena/VASP-like protein in 10 µg of Ramos cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
ABT63 04053252423406