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ABC60 Anti-Endostatin Antibody

Anti-Endostatin Antibody detects level of Endostatin & has been published & validated for use in WB, IH(P).

Anti-Endostatin Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: Col18a1 UniProt Number: P39061
ABC60
100 µg  
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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC60
ReplacesAB1880
DescriptionAnti-Endostatin Antibody
Alternate Names
  • Collagen alpha-1(XVIII) chain
  • Endostatin
Background InformationEndostatin is a proteolytic cleavage product from the C-terminus of Collagen XVIII, which is itself a proteoglycan/collagen found in blood vessel walls and in basement membranes. Endostatin is a compact globular fold-containing protein with a Zn2+ binding site near its amino terminus. Originally identified in conditioned media of murine hemangioendothelioma (EOMA) cells, endostatin is generated from the parent collagen XVIII molecule involving intermediate glycosylated forms and the proteolytic activity of proteases such as cathepsin L, metalloproteases, and elastase. Endostatin has a wide-spread tissue expression and has been shown to co-localize with fibulins, nidogen-2 in basement membranes, and elastic fibers of veins. Endostatin has also been shown to cause a G1 arrest in endothelial cells and to induce apoptosis.
References
Product Information
FormatAffinity Purified
Control
  • A431 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Endostatin Antibody detects level of Endostatin & has been published & validated for use in WB, IH(P).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Endostatin in mouse lung tissue.
Biological Information
ImmunogenLinear peptide corresponding to the nonhelical region 11 of mouse Endostatin.
EpitopeNonhelical Region 11 Domain
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes Endostatin at the nonhelical region 11 domain.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Col18a1
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.

SUBCELLULAR LOCATION: Secreted › extracellular space › extracellular matrix (By similarity).

TISSUE SPECIFICITY: Expressed in liver, kidney, lung, skeletal muscle and testis.

PTM: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

SEQUENCE SIMILARITIES: Belongs to the multiplexin collagen family.

Contains 1 FZ (frizzled) domain.

Contains 1 TSP N-terminal (TSPN) domain.
Molecular Weight~28 kDa observed.
A ~28 kDa endostatin fragment has been observed in western blot as a result of MMP-7 cleavage of collagen XVIII (Chang, J., et al. (2003). Invest Ophthalmol Vis Sci. 44:832).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Endostatin in 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
ABC60 04053252640681

Documentation

Anti-Endostatin Antibody MSDS

职位

物料安全数据表 (MSDS) 

Anti-Endostatin Antibody 分析证书

标题批号
Anti-Endostatin - 2118989 2118989
Anti-Endostatin - 2161183 2161183
Anti-Endostatin - 2266775 2266775
Anti-Endostatin - 2329824 2329824
Anti-Endostatin - 3759979 3759979
Anti-Endostatin - 4201258 4201258
Anti-Endostatin - NRG1953336 NRG1953336
Anti-Endostatin -2737759 2737759
Anti-Endostatin -2803238 2803238
Anti-Endostatin Polyclonal Antibody 3005310
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