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MABC1145 Anti-FAP Antibody, clone 73.3

Detect fibroblast activation protein using this mouse monoclonal Anti-FAP Antibody, clone 73.3, Cat. No. MABC1145. It has been tested in Flow Cytometry.

Anti-FAP Antibody, clone 73.3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: Fap UniProt Number: P97321
MABC1145
100 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1145
DescriptionAnti-FAP Antibody, clone 73.3
Alternate Names
  • Fibroblast activation protein
  • Propyl endopeptidase FAP
  • Dipeptidyl peptidase FAP
  • Serine integral membrane protease
  • SIMP
Background InformationFibroblast activation protein (UniProt: ; also known as Propyl endopeptidase FAP, Dipeptidyl peptidase FAP, Serine integral membrane protease, SIMP) is encoded by the Fap gene (Gene ID: 14089) in murine species. FAP is a homodimeric type II transmembrane protein that physically attaches to cells and bulk of the protein is exposed to extracellular space. It is a gelatinase of the serine protease family that is selectively expressed by tumor associated fibroblasts and pericytes in a majority of human epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. It is not expressed in most normal tissues, but is expressed during embryonic development. It is present as a homodimer and homodimerization is required for activity of both plasma membrane and soluble forms. It can also form heterodimer with DPP4. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences. Its gelatinase activity is inhibited by several serine-protease inhibitors, such as PMSF, AEBSF, and diisopropyl fluorophosphate (DFP). FAP is involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. It is also involved in the cell invasiveness towards the ECM in malignant melanoma cancers. (Ref.: Kelly, T et al. (2012). Int. Rev. Cell Mol. Biol. 297:83-116; Santos, AM et al. (2009). J. Clin. Invest. 119(12):3613-3625).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect fibroblast activation protein using this mouse monoclonal Anti-FAP Antibody, clone 73.3, Cat. No. MABC1145. It has been tested in Flow Cytometry.
Key Applications
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: A representative lot detected FAP in Flow Cytometry applications (Wang, L.C., et. al. (2014). Cancer Immunol Res. 2(2):154-66).

Flow Cytometry Analysis: 1 µg/mL from a representative lot detected FAP in 3T3/FAP overexpressed cells, but not in 3T3 WT cells. (Courtesy of Leslie Hopper, University of Pennsylvania, USA).
Biological Information
ImmunogenFAP-expressing 3T3 cells.
Clone73.3
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 73.3 specifically detects fibroblast activation protein (FAP) in 3T3 cells overexpressing FAP.
IsotypeIgG1κ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Fap
Purification MethodProtein G purified
UniProt Number
Molecular Weight87.94 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.


Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABC1145 04054839169601

Documentation

Anti-FAP Antibody, clone 73.3 MSDS

职位

物料安全数据表 (MSDS) 

Anti-FAP Antibody, clone 73.3 分析证书

标题批号
Anti-FAP, clone 73.3 - 3197663 3197663
Anti-FAP, clone 73.3 - 3291375 3291375
Anti-FAP, clone 73.3 - 3670127 3670127
Anti-FAP, clone 73.3 - 3862615 3862615
Anti-FAP, clone 73.3 - 3874286 3874286
Anti-FAP, clone 73.3 - 3959465 3959465
Anti-FAP, clone 73.3 - 3977212 3977212
Anti-FAP, clone 73.3 - 4070059 4070059
Anti-FAP, clone 73.3 - 4097740 4097740
Anti-FAP, clone 73.3 - 4176612 4176612
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