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ABN1038 Anti-Fbxo7 Antibody

Anti-Fbxo7 Antibody is an antibody against Fbxo7 for use in Western Blotting, Immunohistochemistry, Immunocytochemistry.

Anti-Fbxo7 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: FBXO7, FBX7 UniProt Number: Q9Y3I1
ABN1038
100 μg  
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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IHC, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN1038
DescriptionAnti-Fbxo7 Antibody
Alternate Names
  • F-box only protein 7
  • Fbxo7
Background InformationF-box only protein 7 (UniProt Q9Y3I1) is encoded by the FBXO7 (Also known as FBX, FBX7, PKPS, FBX07, PARK15) gene in human (Gene ID 25793). The Skp1-Cul1-F box protein (SCF) E3 ubiquitin ligase complexes rely on their F-box protein component for substrate recognition. In addition to Fbxs, to which Fbxo7 belongs, there exist two other classes of F-box proteins, Fbws and Fbls. Fbxo7 recruits substrate via its C-terminal proline rich domain and associates with the adapter protein Skp1 via its F-box domain. In addition to mediating SCF-dependent ubiquitination, Fbxo7 is also involved in SCF-independent activities. Fbxo7 is reported to participate in autophagic clearance of damaged mitochondria (mitophagy) in response to mitochondrial depolarisation through direct interaction with PINK1 (PARK6) and Parkin (PARK2), while Parkinson's disease-associated Fbxo7 mutations affect Parkin mitochondrial translocation and Parkin-mediated mitophagy.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-Fbxo7 Antibody is an antibody against Fbxo7 for use in Western Blotting, Immunohistochemistry, Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunocytochemistry
Application NotesImmunohistochemistry Analysis: A 1:400 dilution from a representative lot detected Fbxo7 in human tonsil tissue.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Fbxo7 using U2OS cells transfected with constructs expressing either wildtype (T7-Fbxo7) or a deletion of F-box domain (T7-Fbxo7(ΔFbox)).
Immunohistochemistry Analysis: A representative lot detected Fbxo7 in lung carcinoma and colon adenocarcinoma tissue (Laman, H., et al. (2005). EMBO. 24:3104-3116).
Western Blotting Analysis: A representative lot detected Fbxo7 in U2OS cells, Asynchronous U2OS cells transfected with FBXO7, NIH3T3 cells transfected with Fbxo7(129–522) and FBXO7(deleted FBOX)) (Laman, H., et al. (2005). EMBO. 24:3104-3116).
Western Blotting Analysis: A representative lot detected Fbxo7 in U2OS transfected with Fbxo7, U2OS (Kirk, R., et al. (2008). J. Biol. Chem. 283(32):22325-22335).
Western Blotting Analysis: A representative lot detected Fbxo7 in Ba/F3 transfected with FBXO7,Ba/F3, Nalm-6, C-33 A, mouse spleen, liver, and cerebellum (Meziane, E.K., et al. (2011). J. Cell. Science. 124:2175-2186).
Western Blotting Analysis: A representative lot detected Fbxo7 in U2OS transfected with FBXO7,Hek293, and SH-SY5Y (Burchell, V.S., et al. (2013). Nature Neuroscience. 16(9):1257-1265).
Immunocytochemistry Analysis: A representative lot detected Fbxo7 in U2OS cells (Kirk, R., et al. (2008). J. Biol. Chem. 283(32):22325-22335).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human Fbxo7.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityExpected to cross react to all isoforms.
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • FBXO7
  • FBX7
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~65 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Fbxo7 in 10 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
ABN1038 04055977169942