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MABC1743-100UL Anti-KCNK9 Antibody, clone Y4

Anti-KCNK9, clone Y4, Cat. No. MABC1743, is a mouse monoclonal antibody that detects Potassium channel subfamily K member 9 and is tested for use in Flow Cytometry, Function Analysis, Inhibition Assay, and Western Blotting.

MABC1743-100UL
100 µL  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberMABC1743-100UL
DescriptionAnti-KCNK9 Antibody, clone Y4
Alternate Names
  • Potassium channel subfamily K member 9
  • Acid-sensitive potassium channel protein TASK-3
  • TWIK-related acid-sensitive K(+) channel 3
  • Two pore potassium channel KT3.2
  • Two pore K(+) channel KT3.2
Background InformationPotassium channel subfamily K member 9 (UniProt: Q9NPC2; also known as Acid-sensitive potassium channel protein TASK-3, TWIK-related acid-sensitive K(+) channel 3, Two pore potassium channel KT3.2, Two pore K(+) channel KT3.2, KCNK9) is encoded by the KCNK9 (also known as TASK3) gene (Gene ID: 51305) in human. KCNK9 is a homodimeric multi-pass membrane protein of the two-pore domain potassium channel that maintains resting membrane potential of the cell. It is highly sensitive to changes in pH. It can also form heterodimer with KCNK1. It is found mainly in cerebellum, but has also been detected in adrenal gland, kidney, and lung. Over-expression of KCNK9 has been reported in several carcinomas, including breast, lung, colon, and prostate. It is reported to confer resistance to growth factor deprivation and hypoxia, which promotes tumor cell survival in hypoxic areas of solid tumors. KCNK9 has three cytoplasmic domains (aa 1-8, 129-158, and 240-374), four transmembrane domains, four extracellular domains, and two pore-forming domains (aa 89-101 and 195-207). Clone Y4 displays highest affinity for KCNK9 (KD = 0.7 nM) and induces channel internalization. Addition of Y4 to KCNK9-expressing carcinoma cells is shown to reduce their viability and increase cell death. It also inhibits the growth of human lung cancer xenografts and murine breast cancer metastasis in mice. (Ref.: Sun, H., et al. (2016). Nat. Commun. 7; 10339).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide
Quality LevelMQ200
Applications
ApplicationAnti-KCNK9, clone Y4, Cat. No. MABC1743, is a mouse monoclonal antibody that detects Potassium channel subfamily K member 9 and is tested for use in Flow Cytometry, Function Analysis, Inhibition Assay, and Western Blotting.
Key Applications
  • Flow Cytometry
  • Function Assay
  • Inhibition
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected KCNK9 in Western Blotting applications (Sun, H., et. al. (2016). Nat Commun. 7:10339).

Flow Cytometry Analysis: 10 ug per million cells from a representative lot detected KCNK9 in BEN cells (Courtesy of John Laterra, M.D., Ph.D., Kennedy Krieger Institute, John Hopkins Hospital, Baltimore, MD, USA).

Inhibits Activity/Function: A representative lot inhibited growth of human lung cancer xenografts and murine breast cancer metastasis in mice. (Sun, H., et. al. (2016). Nat Commun. 7:10339).

Flow Cytometry Analysis: A representative lot detected KCNK9 in Flow Cytometry applications (Sun, H., et. al. (2016). Nat Commun. 7:10339).

Affects Function: A representative lot induced endocytosis of functional channels. (Sun, H., et. al. (2016). Nat Commun. 7:10339).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenRecombinant fragment corresponding to the 59 amino acids from the first extracellular domain (M1P1 loop) of human Potassium channel subfamily K member 9 (KCNK9).
Epitopeextracellular domain
CloneY4
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone Y4 is a mouse monoclonal antibody that detects human Potassium channel subfamily K member 9 (KCNK9). It targets an epitope within 59 amino acids from the first extracellular loop.
IsotypeIgG1
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • KCNK9
  • TASK3
Purification MethodProtein G purified
UniProt Number
Molecular Weight42.26 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in BT-549 cells.

Flow Cytometry Analysis: A 1:50 dilution of this antibody detected KCNK9 in one million BT-549 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABC1743-100UL 04061842414567

Documentation

Anti-KCNK9 Antibody, clone Y4 MSDS

职位

物料安全数据表 (MSDS) 

Anti-KCNK9 Antibody, clone Y4 分析证书

标题批号
Anti-KCNK9, clone Y4 - Q3495042 Q3495042

参考

参考概述公共医疗ID
A monoclonal antibody against KCNK9 K(+) channel extracellular domain inhibits tumour growth and metastasis
Han Sun 1 2 , Liqun Luo 3 , Bachchu Lal 2 , Xinrong Ma 4 , Lieping Chen 5 , Christine L Hann 6 , Amy M Fulton 4 7 , Daniel J Leahy 8 , John Laterra 1 2 6 9 , Min Li
Nat Commun  7  10339  2016

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