Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABF3201-25UL Anti-MART-1/Melan-A Antibody, clone A103

Anti-MART-1/Melan-A, clone A103, Cat. No. MABF3201, is a mouse monoclonal antibody that detects MART-1 and is tested for use in Immunohistochemistry and Western Blotting.

MABF3201-25UL
25 µL  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberMABF3201-25UL
DescriptionAnti-MART-1/Melan-A Antibody, clone A103
Alternate Names
  • Melanoma antigen recognized by T-cells 1
  • Antigen LB39-AA
  • Antigen SK29-AA
  • Protein Melan-A
Background InformationMelanoma antigen recognized by T-cells 1 (UniProt: Q16655; also known as MART-1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A) is encoded by the MLANA (also known as MART1) gene (Gene ID: 2315) in human. MART-1, a melanosome-specific protein, is a single-pass type III membrane protein with a transmembrane domain (aa 27-47) and a cytoplasmic domain (aa 48-118). Its expression is restricted to melanocytes, melanoma, and retina and is absent in other tissues and tumors. It is highly enriched in early melanosomes (Stage I and/or II melanosomes) indicating that its role in early melanogenesis. It is reported to stabilize both GPCR143/OA-1 and plays a role in the expression, stability, trafficking, and processing of pre-melanosome protein (PMEL) to ensure the formation of a stage 2 melanosome. It is mainly found in the endoplasmic reticulum (ER) and Golgi but is also detected in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation, which allows melanoma cells to be effectively recognized by specific cytotoxic T-cells. MART-1 may be a useful marker in the diagnosis of melanocytic lesions and in the differential diagnosis of malignant melanoma. (Ref.: Hoashi, T., et al. (2005). J. Biol. Chem. 280(14); 14006-14016; Chen, YT., et al. (1996). Proc. Natl. Acad. Sci. USA. 93(12); 5915-5919).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-MART-1/Melan-A, clone A103, Cat. No. MABF3201, is a mouse monoclonal antibody that detects MART-1 and is tested for use in Immunohistochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesTested applications

Western Blotting Analysis: A representative lot detected MART-1 in Western Blotting applicaiton. (Chen, Y.T., et al. (1996). Proc Natl Acad Sci USA. 93(12); 5915-9).

Immunohistochemistry Applications: A representative lot detected MART-1/Melan-A in Immunohistochemistry applications (Chen, Y.T., et al. (1996). Proc Natl Acad Sci USA. 93(12);5915-9).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenFull-length, recombinant, human MART-1 produced in E.coli.
EpitopeUnknown
CloneA103
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone A103 is a mouse monoclonal antibody that detects MART-1.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MLANA
Purification MethodProtein G purified
UniProt Number
Molecular Weight~16 kDa observed; 13.16 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in SK-MEL-28 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected MART-1 in SK-MEL-28 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABF3201-25UL 04065269800194