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IM33 Anti-MMP-2 (Ab-3) Mouse mAb (42-5D11)

This Anti-MMP-2 (Ab-3) Mouse mAb (42-5D11) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of MMP-2 (Ab-3).

Anti-MMP-2 (Ab-3) Mouse mAb (42-5D11) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

同义词: Anti-72 kDa Gelatinase, Anti-Gelatinase A, Anti-Matrix Metalloproteinase 2

IM33
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityHostAntibody Type
B, H, M, RMMonoclonal Antibody

Products

产品目录编号包装 数量 / 包装
IM33-100UGCN 塑胶安瓿;塑胶针药瓶 100 μg
Description
OverviewRecognizes the ~72 kDa latent and the ~66 kDa active forms of the MMP-2.
Catalogue NumberIM33
Brand Family Calbiochem®
SynonymsAnti-72 kDa Gelatinase, Anti-Gelatinase A, Anti-Matrix Metalloproteinase 2
Application Data
Detection of human MMP-2 by immunoblotting. Sample: Conditioned medium of normal human skin fibroblasts NB1RGB cells. Primary antibody: Anti-MMP-2 (Ab-3) Mouse mAb (42-5D11) (Cat. No. IM33) (2 µg/ml). Detection: chemiluminescence.
References
ReferencesCottam, D. W. and Rees, R. C. 1993. Intl J. Oncol. 2, 861.
Fujimoto, N., et al. 1993. Clinica Chimica Acta. 221, 91.
Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
Woessner, J. F. 1991. FASEB J. 5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 99.
Product Information
DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
FormLiquid
FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0
Negative controlMMP-9 protein (Cat. Nos. PF024 or PF038)
Positive controlMMP-2 protein (Cat. Nos. PF023 or PF037)
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesFrozen Sections Nakagawa, T., et al. 1994. J. Neurosurg. 81, 69. Immunoblotting, Original Clone Fujimoto, N., et al. 1993. Clinica Chimica Acta. 221, 91. Paraffin Sections Tsuchiya, Y., et al. 1994. Int. J. Cancer 56, 46.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesFrozen Sections (see application references)
Immunoblotting (1 µg/ml)
Paraffin Sections (see application references)
Application CommentsThis antibody will not cross-react with human MMP-1, MMP-3, MMP-9, or MMP-13. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (VTPRDKPMGPLLVATF) corresponding to amino acids 468-483 of human MMP-2
ImmunogenHuman
Epitopewithin amino acids 468-483 of human MMP-2 (numbered from the propeptide)
Clone42-5D11
HostMouse
IsotypeIgG₁
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
IM33-100UGCN 07790788053772

Documentation

Anti-MMP-2 (Ab-3) Mouse mAb (42-5D11) MSDS

职位

物料安全数据表 (MSDS) 

Anti-MMP-2 (Ab-3) Mouse mAb (42-5D11) 分析证书

标题批号
IM33

参考

参考信息概述
Cottam, D. W. and Rees, R. C. 1993. Intl J. Oncol. 2, 861.
Fujimoto, N., et al. 1993. Clinica Chimica Acta. 221, 91.
Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
Woessner, J. F. 1991. FASEB J. 5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 99.

小册子

标题
Angiogenesis and Tumor Metastasis Brochure and Technical Guide
数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-July-2007 JSW
SynonymsAnti-72 kDa Gelatinase, Anti-Gelatinase A, Anti-Matrix Metalloproteinase 2
ApplicationFrozen Sections (see application references)
Immunoblotting (1 µg/ml)
Paraffin Sections (see application references)
Application Data
Detection of human MMP-2 by immunoblotting. Sample: Conditioned medium of normal human skin fibroblasts NB1RGB cells. Primary antibody: Anti-MMP-2 (Ab-3) Mouse mAb (42-5D11) (Cat. No. IM33) (2 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody (see application references). Recognizes the ~72 kDa latent and the ~66 kDa active forms of MMP-2.
BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin, and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an approximately 10 kDa segment from the N-terminus, and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. MMP-2 (Gelatinase A, 72 kDa gelatinase/type IV collagenase, TBE-1) is secreted as a 72 kDa zymogen which is proteolytically processed to the 66 kDa active form. MMP-2, along with its most closely related member of the MMP family, MMP-9, show substrate specificity toward type IV and V collagens, gelatin, and elastin. Numerous studies have shown a correlation between collagenase expression and metastatic potential, and suggest that it may be a useful marker for the diagnosis or prognosis of cancer.
HostMouse
Immunogen speciesHuman
Immunogena synthetic peptide (VTPRDKPMGPLLVATF) corresponding to amino acids 468-483 of human MMP-2
Epitopewithin amino acids 468-483 of human MMP-2 (numbered from the propeptide)
Clone42-5D11
IsotypeIgG₁
Speciesbovine, human, mouse, rat
Positive controlMMP-2 protein (Cat. Nos. PF023 or PF037)
Negative controlMMP-9 protein (Cat. Nos. PF024 or PF038)
FormLiquid
FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody will not cross-react with human MMP-1, MMP-3, MMP-9, or MMP-13. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesCottam, D. W. and Rees, R. C. 1993. Intl J. Oncol. 2, 861.
Fujimoto, N., et al. 1993. Clinica Chimica Acta. 221, 91.
Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
Woessner, J. F. 1991. FASEB J. 5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 99.
Application referencesFrozen Sections Nakagawa, T., et al. 1994. J. Neurosurg. 81, 69. Immunoblotting, Original Clone Fujimoto, N., et al. 1993. Clinica Chimica Acta. 221, 91. Paraffin Sections Tsuchiya, Y., et al. 1994. Int. J. Cancer 56, 46.