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IM36 Anti-MMP-3 (Ab-1) Mouse mAb (55-2A4)

This Anti-MMP-3 (Ab-1) Mouse mAb (55-2A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of MMP-3 (Ab-1).

Anti-MMP-3 (Ab-1) Mouse mAb (55-2A4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
IM36
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityHostAntibody Type
H, Porcine, R, ShMMonoclonal Antibody

Products

产品目录编号包装 数量 / 包装
IM36-100UGCN Plastic Bag(s) 100 μg
Description
OverviewRecognizes the ~57/59 kDa latent and the ~28/45 kDa active forms of MMP-3. Will also recognize MMP-3/TIMP-1 complexes. Slightly cross-reacts (~34%) with human MMP-10.
Catalogue NumberIM36
Brand Family Calbiochem®
Application Data
Detection of human MMP-3 by immunoblotting. Sample: Conditioned medium of normal human skin fibroblasts NB1RGB cells. Primary antibody: Anti-MMP-3 (Ab-1) Mouse mAb (55-2A4) (Cat. No. IM36) (0.5 µg/ml). Detection: chemiluminescence.
References
ReferencesCottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 99.
Product Information
DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
FormLiquid
FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesFrozen Sections (see application references)
Immunoblotting (1 µg/ml)
Paraffin Sections (see comments and application references)
Application CommentsThis antibody has 34% cross-reactivity with human MMP-10. For paraffin sections, staining can be enhanced by culturing tissue in 2 µM monensin for 3 h. Pre-treating with trypsin or a pressure cooker may enhance staining. Tissue can then be fixed with PLP (perioddate-lysine-4% paraformaldehyde) and stained using 5-10 µg/ml MMP-3 antibody. See application references for more information. Clone 55-2A4 is called Clone B in Obata, K., et al. To prepare conditioned medium for positive control, incubate HT-1080 cells for 2 h at 37°C in serum-free media containing 100 nM PMA. Collect and centrifuge medium; concentrate as necessary. Store in aliquots at -20°C. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenhuman pro-MMP-3 from the conditioned media of rheumatoid synovial fibroblasts
ImmunogenHuman
Clone55-2A4
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Porcine
  • Rat
  • Sheep
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
IM36-100UGCN 04055977220575

Documentation

Anti-MMP-3 (Ab-1) Mouse mAb (55-2A4) MSDS

职位

物料安全数据表 (MSDS) 

Anti-MMP-3 (Ab-1) Mouse mAb (55-2A4) 分析证书

标题批号
IM36

参考

参考信息概述
Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 99.
数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-July-2007 JSW
ApplicationFrozen Sections (see application references)
Immunoblotting (1 µg/ml)
Paraffin Sections (see comments and application references)
Application Data
Detection of human MMP-3 by immunoblotting. Sample: Conditioned medium of normal human skin fibroblasts NB1RGB cells. Primary antibody: Anti-MMP-3 (Ab-1) Mouse mAb (55-2A4) (Cat. No. IM36) (0.5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody (see application references). Recognizes the ~59/57 kDa latent and the ~45/28 kDa active forms of MMP-3 and MMP-3/TIMP complexes (see application references).
BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an approximately 10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. MMP-3 (stromelysin-1) is secreted as a 59/57 kDa zymogen that is proteolytically processed to the 45 and 28 kDa active forms. This enzyme degrades a wide spectrum of extracellular matrix proteins, including cartilage proteoglycans, fibronectin, type IV and IX collagen and laminin. MMP-3 is thought to play an important role in pathophysiological degradation processes associated with conditions such as rheumatoid arthritis and cancer cell invasion.
HostMouse
Immunogen speciesHuman
Immunogenhuman pro-MMP-3 from the conditioned media of rheumatoid synovial fibroblasts
Clone55-2A4
IsotypeIgG₁
Speciesnot bovine, human, not mouse, porcine, not rabbit, rat, sheep
FormLiquid
FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody has 34% cross-reactivity with human MMP-10. For paraffin sections, staining can be enhanced by culturing tissue in 2 µM monensin for 3 h. Pre-treating with trypsin or a pressure cooker may enhance staining. Tissue can then be fixed with PLP (perioddate-lysine-4% paraformaldehyde) and stained using 5-10 µg/ml MMP-3 antibody. See application references for more information. Clone 55-2A4 is called Clone B in Obata, K., et al. To prepare conditioned medium for positive control, incubate HT-1080 cells for 2 h at 37°C in serum-free media containing 100 nM PMA. Collect and centrifuge medium; concentrate as necessary. Store in aliquots at -20°C. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesCottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 99.