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MABS496 Anti-Mineralocorticoid Receptor Antibody, clone 6G1

Detect Mineralocorticoid Receptor using this Anti-Mineralocorticoid Receptor Antibody, clone 6G1 validated for use in Western Blotting, Immunohistochemistry and Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: Nr3c2, Mlr UniProt Number: P22199
MABS496
100 μL  
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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, MWB, IHC, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS496
DescriptionAnti-Mineralocorticoid Receptor Antibody, clone 6G1
Alternate Names
  • MR
  • Nuclear receptor subfamily 3 group C member 2
  • Mineralocorticoid Receptor
Background InformationMineralocorticoid receptor (UniProt P22199; also known as MR, Nuclear receptor subfamily 3 group C member 2) is encoded by the Nr3c2 (also known as MCR, Mlr) gene (Gene ID 25672) in rats. Mineralocorticoid receptor (MR) is expressed in multiple tissues, including renal epithelia, smooth muscle, endothelium, cardiomyocytes, and hippocampal neurons, where it mediates diverse functions. MR is activated by aldosterone and also by cortisol in cells that do not express 11β-hydroxysteroid dehydrogenase type 2 (11βHSD2). MR is normally activated by aldosterone, which is produced by adrenal glomerulosa in response to intravascular volume depletion and hyperkalemia. Consistently, gain of function mutations in MR lead to severe hypertension, often with hypokalemia, while loss of function mutations result in neonatal hypotension. Ser843 phosphorylation in the MR ligand-binding domain is reported to prevent MR ligand binding and activation. MR pS843 is found exclusively in intercalated cells of the distal nephrons in the kidney. Intravascular volume depletion, angiotensin II and WNK4 signaling reduce MR pS843 levels, whereas hyperkalemia increases MR pS843.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Mineralocorticoid Receptor using this Anti-Mineralocorticoid Receptor Antibody, clone 6G1 validated for use in Western Blotting, Immunohistochemistry and Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunocytochemistry
Application NotesImmunohistochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in various rat tissues including kidney, hippocampus, choroid plexus, cerebellum, colon, heart, and coronary artery (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348).
Immunocytochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in mouse kidney cells (Shibata, S., et al. (2013). Cell Metabolism. 18:660-671).
Western Blotting Analysis: A representative lot detected Mineralocorticoid Receptor in rat hippocampal tissue lysate (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348).
Western Blotting Analysis: A representative lot detected endogenous Mineralocorticoid Receptor (MR) in mouse kidney and exogenously expressed human MR in transfected COS-7 cells (Shibata, S., et al. (2013) Cell Metab. 18(5):660-671).
Biological Information
ImmunogenConjugated linear peptide corresponding to rat Mineralocorticoid Receptor.
Clone6G1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityEpitope is present in all 3 alternative spliced isoforms.
IsotypeIgG1
Species Reactivity
  • Rat
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Nr3c2
  • Mlr
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~107 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in M1MR cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Mineralocorticoid Receptor in 10 µg of M1MR cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABS496 04055977173147

Documentation

Anti-Mineralocorticoid Receptor Antibody, clone 6G1 MSDS

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物料安全数据表 (MSDS) 

Anti-Mineralocorticoid Receptor Antibody, clone 6G1 分析证书

标题批号
Anti-Mineralocorticoid Receptor, -Q2573226 Q2573226
Anti-Mineralocorticoid Receptor, clone 6G1 - 3172403 3172403
Anti-Mineralocorticoid Receptor, clone 6G1 - 3237523 3237523
Anti-Mineralocorticoid Receptor, clone 6G1 - 3660368 3660368
Anti-Mineralocorticoid Receptor, clone 6G1 - 3841820 3841820
Anti-Mineralocorticoid Receptor, clone 6G1 - 3940936 3940936
Anti-Mineralocorticoid Receptor, clone 6G1 - 4095406 4095406
Anti-Mineralocorticoid Receptor, clone 6G1 - 4189652 4189652
Anti-Mineralocorticoid Receptor, clone 6G1 - 4249189 4249189
Anti-Mineralocorticoid Receptor, clone 6G1 Monoclonal Antibody 3128832
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技术信息

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White Paper: Further considerations of antibody validation and usage.