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05-716-I-25UL Anti-Myosin Heavy Chain Antibody, clone A4.1025

Anti-Myosin Heavy Chain, clone A4.1025, Cat. No. 05-716-I, is a mouse monoclonal antibody that detects Myosin Heavy Chain and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

05-716-I-25UL
25 µL  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number05-716-I-25UL
DescriptionAnti-Myosin Heavy Chain Antibody, clone A4.1025
Alternate Names
  • Myosin-1
  • Myosin heavy chain 1
  • Myosin heavy chain 2x
  • MyHC-2x
  • Myosin heavy chain IIx/d
  • MyHC-IIx/d
  • Myosin heavy chain skeletal muscle adult 1
Background InformationMyosin-1 (UniProt: P12882; also known as Myosin heavy chain 1, Myosin heavy chain 2x, MyHC-2x, Myosin heavy chain IIx/d, MyHC-IIx/d, Myosin heavy chain skeletal muscle adult 1) is encoded by the MYH1 gene (Gene ID: 4619) in human. Myosins are highly conserved, ubiquitously expressed proteins that form regular bipolar thick filaments that together with actin thin filaments constitute the fundamental contractile unit of skeletal and cardiac muscle. Along with actin they serve as motor molecules with ATPase activity that is essential for muscle contraction. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular sub-fragments (S1) and 1 rod-shaped sub-fragment (S2). The globular head of myosin heavy chain contains the actin-activated ATPase. It has been reported that during rest, the myosin head does interact with the actin filament due to the presence of tropomyosin. However, when muscle contracts, tropomyosin shifts out of the way to allow the globular head to engage the actin filaments. Myosin heavy chain is a conventional myosin with a N-terminal SH3-like domain (aa 37-82), a myosin motor domain (aa 86-782), and an actin-binding domain (aa 761-775). It has a rod like tail sequence that is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Myosin Heavy Chain, clone A4.1025, Cat. No. 05-716-I, is a mouse monoclonal antibody that detects Myosin Heavy Chain and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Myosin Heavy Chain in lysates from L6 cells and human skeletal muscle.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Myosin Heavy Chain in human skeletal muscle tissue sections.

Immunohistochemistry Applications: A representative lot detected Myosin Heavy Chain in Immunohistochemistry applications (Laternser, S., et. al. (2018). SLAS Technol. 23(6):599-613).

Western Blotting Analysis: A representative lot detected Myosin Heavy Chain in Western Blotting applications (Jingxuan, Li., et. al. (2020). Genes (Basel). 11(3):259).

Immunofluorescence Analysis: A representative lot detected Myosin Heavy Chain in Immunofluorescence applications (Jingxuan, Li., et. al. (2020). Genes (Basel). 11(3):259).

Immunocytochemistry Analysis: A representative lot detected Myosin Heavy Chain in Immunocytometry applications (Srikuea, R., et. al. (2020). Sci Rep. 10(1):8239).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenPartially purified myosin from adult human skeletal muscle.
EpitopeUnknown
CloneA4.1025
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone A4.1025 is a mouse monoclonal antibody that detects Myosin Heavy Chain.
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Drosophila, Rabbit, Zebra Fish based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MYH1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~220 kDa observed; 223.15 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in C2C12 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Myosin Heavy Chain in C2C12 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
05-716-I-25UL 04065265324236

Documentation

Anti-Myosin Heavy Chain Antibody, clone A4.1025 MSDS

职位

物料安全数据表 (MSDS) 

Anti-Myosin Heavy Chain Antibody, clone A4.1025 分析证书

标题批号
Anti-Myosin Heavy Chain, clone A4.1025 - 3985008 3985008
Anti-Myosin Heavy Chain, clone A4.1025 - 4076631 4076631
Anti-Myosin Heavy Chain, clone A4.1025 - 4211802 4211802
Anti-Myosin Heavy Chain, clone A4.1025 - 4284748 4284748
Anti-Myosin Heavy Chain, clone A4.1025 - 4306299 4306299
Anti-Myosin Heavy Chain, clone A4.1025 - Q3543330 Q3543330