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MABT496 Anti-PKN2, clone 1D1 Antibody

Anti-PKN2, clone 1D1 Antibody is an antibody against PKN2 for use in western blotting, ELISA, IHC, flow cytometry.

Anti-PKN2, clone 1D1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: PKN2, PRK2, PRKCL2 UniProt Number: Q16513
MABT496
100 µL  
Purchase on Sigma-Aldrich

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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, M, MkWB, ELISA, IHC, FCMAscitesMonoclonal Antibody
Description
Catalogue NumberMABT496
DescriptionAnti-PKN2, clone 1D1 Antibody
Alternate Names
  • Serine/threonine-protein kinase N2
  • Protein kinase C-like 2
  • Protein-kinase C-related kinase 2
  • PKN2
  • PRK2
  • PRKCL2
Background InformationSerine/threonine-protein kinase N2, also known as Protein kinase C-like 2 or Protein-kinase C-related kinase 2, and encoded by the gene name PKN2/PRK2/PRKCL2, is a PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. PKN2 has been shown to play an important role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Protein-kinase-C-related kinases (PRKs) are part of the lipid-regulated protein kinases (PKC) which also include liver PAK & PKN. Human PRK1 and PRK2 share structurally similar catalytic domains, but less similar N-terminal regulatory regions suggesting different regulatory domain functions. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues. Additionally, the serine-threonine kinase PRK2 can be specifically cleaved by caspase-3 (and/or caspase-3-like subfamily members) during apoptosis. PKN2 is expressed in numerous tumor cell lines, especially in bladder tumor cells.
References
Product Information
FormatAscites
PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PKN2, clone 1D1 Antibody is an antibody against PKN2 for use in western blotting, ELISA, IHC, flow cytometry.
Key Applications
  • Western Blotting
  • ELISA
  • Immunohistochemistry
  • Flow Cytometry
Application NotesELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

Immunohistochemistry Analysis: A 1;200-1,000 dilution from a representative lot detected PKN2 in prostate and lung cancer tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected PKN2 in NIH/3T3 cells.
Biological Information
ImmunogenPurified recombinant fragment of human PKN2 expressed in E. Coli
Clone1D1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
Species Reactivity
  • Human
  • Rat
  • Mouse
  • Monkey
Antibody TypeMonoclonal Antibody
Gene Symbol
  • PKN2
  • PRK2
  • PRKCL2
Purification MethodUnpurified
UniProt Number
Molecular Weight~140 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in PC-12, Cos7, K562, Jurkat, HeLa, A431, C6, NIH/3T3 and HEK293 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PKN2 in PC-12, Cos7, K562, Jurkat, HeLa, A431, C6, NIH/3T3 and HEK293 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABT496 04053252955501