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ABF1065-25UL Anti-SARS-CoV-2 spike protein S1 domain

Anti-SARS-CoV-2 spike protein S1 domain, Cat. No. ABF1065, is a rabbit polyclonal antibody that detects the S1 domain of SARS-CoV-2 spike protein and is tested for use in ELISA, Neutralizing, and Western Blotting.

ABF1065-25UL
25 µL  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberABF1065-25UL
DescriptionAnti-SARS-CoV-2 spike protein S1 domain
Alternate Names
  • Spike glycoprotein
  • S glycoprotein
  • E2
  • Peplomer protein
  • Spike protein S1 domain
Background InformationSpike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion. This polyclonal antibody binds and neutralizes the SARS-CoV-2 variants including the B.1.1.7; B.1.429; P.1; and B.1.351 at different levels. (Ref.: Ravichandran, S., et al. (2020). Sci Transl Med. 12(550):eabc3539).
References
Product Information
FormatSerum
PresentationRabbit polyclonal antibody in serum without preservatives.
Quality LevelMQ200
Applications
ApplicationAnti-SARS-CoV-2 spike protein S1 domain, Cat. No. ABF1065, is a rabbit polyclonal antibody that detects the S1 domain of SARS-CoV-2 spike protein and is tested for use in ELISA, Neutralizing, and Western Blotting.
Key Applications
  • Western Blotting
  • Enzyme Immunoassay (ELISA)
  • Neutralizing
Application NotesEnzyme Immunoassay Analysis: A representative lot detected SARS-CoV-2 spike protein S1 domain in ELISA applications (Ravichandran, S., et. al. (2020). Sci Transl Med. 12(550):eabc3539).

Neutralizing: A representative lot neutralized SARS-CoV-2 spike protein S1 domain in Neutralizing applications (Ravichandran, S., et. al. (2020). Sci Transl Med. 12(550):eabc3539).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to the S1 domain (aa 16-685) from the extracellular region of SARS-CoV-2 spike protein.
EpitopeN-terminal half
ConcentrationPlease refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects the S1 domain of SARS-CoV-2 spike protein.
IsotypeIgG
Species Reactivity
  • Virus
Species Reactivity NoteVirus
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • S
Purification MethodUnpurified
UniProt Number
Molecular Weight38 kDa and 170 kDa observed for RBD and complete extracellular domain. 139.13 kDa calculated for spike protein. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant S1 domain and the extracellular domain of SARS-CoV-2 spike protein.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant S1 domain and the complete extracellular domain of SARS-CoV-2 spike protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
ABF1065-25UL 04065267022505