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MABE1128 Anti-SETBP1 Antibody, clone 63

Anti-SETBP1, clone 63, Cat. No. MABE1128, is a highly specific mouse monoclonal antibody that targets SETBP1 and has been tested in Western Blotting.

Anti-SETBP1 Antibody, clone 63 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: SETBP1, KIAA0437, MRD29 UniProt Number: Q9Y6X0
MABE1128
100 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1128
DescriptionAnti-SETBP1 Antibody, clone 63
Alternate Names
  • SET-binding protein
  • SEB
Background InformationSET-binding protein (UniProt Q9Y6X0; also known as SEB, SETBP1) is encoded by the SETBP1 (also known as KIAA0437, MRD29) gene (Gene ID 26040) in human. SETBP1 is shown to bind the SET nuclear oncogene involved in DNA replication. It is expressed in various tissues with low levels in myeloid and monocytic cells and in CD34+ cells, however, higher expression is observed in myeloid malignancies. Overexpression of SETBP1 promotes leukemogenesis by enhancing full-length SET protein and then impairing the phosphatase activity of the tumor suppressor PP2A through the formation of a SETBP1-SET-PP2A complex. Patients with SETBP1 overexpression have a significantly shorter overall survival, and poor prognosis, particularly in the elderly patients. Mutations in SETBP1 gene are associated with Schinzel-Giedion midface retraction syndrome, which is characterized by severe mental retardation, distinctive facial features, and multiple congenital malformations. (Ref.: Cristobal, I., et al. (2010). Blood 115(3): 615-625).
References
Product Information
FormatPurified
PresentationPurified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-SETBP1, clone 63, Cat. No. MABE1128, is a highly specific mouse monoclonal antibody that targets SETBP1 and has been tested in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:4 dilution of clone 63 hybridoma culture supernatant detected SETBP1 in 30-50 µg of REH human acute lymphoid leukemia cell lysate (Courtesy of Jesper Christensen, Ph.D. and Kristian Helin, Ph.D., University of Copenhagen, Denmark).
Biological Information
ImmunogenGST-tagged recombinant human SETBP1 N-terminal fragment.
Clone63
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 63 targets the N-terminal region of human and mouse SETBP1.
IsotypeIgG2bκ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SETBP1
  • KIAA0437
  • MRD29
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~175 kDa observed. 175.0 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SETBP1 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABE1128 04054839134333