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ABS527 Anti-Sphk2, C-term Antibody

ABS527
100 µL  
Purchase on Sigma-Aldrich

特卖

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWB, IP, ICCRbSerumPolyclonal Antibody
Description
Catalogue NumberABS527
DescriptionAnti-Sphk2, C-term Antibody
Alternate Names
  • Sphingosine kinase 2
  • SK 2
  • SPK 2
Background InformationSphingosine kinase 2 (Sphk2), along with its sister enzyme Sphk1, is an important kinase needed to form the important intra and extracellular signaling lipid mediator and neuroprotective factor SPP/S1P (sphingosine 1-phosphate). Sphk2 is important in blood vessel development, brain development, as well as an anti-apoptotic factor, and it mediates many proliferation mediated responses such as those surrounding insulin and its mitogenic ability. Sphk2 is localized to the lysosome membrane primarily but can also be found in the cytoplasm. Interestingly, recent research indicates that ApoE regulates the secretion Sphingosine 1-phosphate in the brain, and SPP/S1P in turn positively regulates glutamate secretion and synaptic strength in hippocampal neurons, thus Sphk1/2 plays an important role in synaptic plasticity and neuronal activity connectivity in the brain as well.
References
Product Information
FormatSerum
PresentationRabbit polyclonal serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Sphk2, C-term Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of Sphk2, C-term.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Sphk2 in 10 µg of mouse lung tissue lysate.
Western Blotting Analysis: A representative lot detected Sphk2 in mouse BMMC tissue lysate (Olivera, A., et al. (2006). JBC. 281(5):2515-2525).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Sphk2 in mouse BMMC tissue lysate (Olivera, A., et al. (2006). JBC. 281(5):2515-2525).
Immunocytochemistry Analysis: A representative lot detected Sphk2 in Huvec cells (Venkataraman, K., et al. (2006) Biochem. J. 397:461-471).
Biological Information
ImmunogenRecombinant protein corresponding to the C-terminus of mouse Sphk2, C-term.
EpitopeC-terminus
HostRabbit
Species Reactivity
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Sphk2
Purification MethodUnpurified
UniProt Number
Molecular Weight~65 kDa observed, uncharacterized bands may be observed at higher molecular weights
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Sphk2, C-term in 10 µg of RAW264.7 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
ABS527 04055977097092

Documentation

Anti-Sphk2, C-term Antibody MSDS

职位

物料安全数据表 (MSDS) 

Anti-Sphk2, C-term Antibody 分析证书

标题批号
Anti-Sphk2, C-term - Q2459306 Q2459306
Anti-Sphk2, C-term Polyclonal Antibody 3140202

参考

参考概述公共医疗ID
IgE-dependent activation of sphingosine kinases 1 and 2 and secretion of sphingosine 1-phosphate requires Fyn kinase and contributes to mast cell responses.
Olivera, Ana, et al.
J. Biol. Chem., 281: 2515-25 (2006)  2006

显示摘要
16316995 16316995
Extracellular export of sphingosine kinase-1a contributes to the vascular S1P gradient.
Venkataraman, Krishnan, et al.
Biochem. J., 397: 461-71 (2006)  2006

显示摘要
16623665 16623665