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AB1543P Anti-Synapsin I Antibody

AB1543P
10 µg  
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      概述

      Replacement Information

      重要规格表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, RELISA, ICC, IHC, IH(P), IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1543P
      ReplacesMAB355
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Synapsin I Antibody
      Alternate Names
      • Synapsin-1
      Background InformationThe synapsins are a family of proteins that have long been implicated in the regulation of neurotransmitter release at synapses. Specifically, they are thought to be involved in regulating the number of synaptic vesicles available for release via exocytosis at any one time. Synapsins are encoded by three different genes, synapsin I, II and III, and different neuron terminals will encode different amounts of these; synapsin will make up 1% of total brain protein at any one time.
      Current studies suggest the following hypothesis for the role of synapsin: synapsins bind synaptic vesicles to components of the cytoskeleton which prevents them from migrating to the presynaptic membrane and releasing transmitter. During an action potential, synapsins are phosphorylated by Ca2+/calmodulin-dependent protein kinase II, releasing the synaptic vesicles and allowing them to move to the membrane and release their neurotransmitter.
      References
      Product Information
      FormatPurified
      Control
      • Brain tissue.
      PresentationPurified rabbit polyclonal in buffer lyophilized from 5 mM ammonium bicarbonate. Reconstitute with 50 μL of PBS.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Synapsin I Antibody is validated for use in ELISA, IC, IH, IH(P), IP, WB for the detection of Synapsin I.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern blotting:
      1:200-1:1,000 dilution of a previous lot was used.

      Immunocytochemistry:
      1:500-1:2,000 dilution of a previous lot was used.

      Immunoprecipitation:
      1 μg of a previous lot immunoprecipitated all of the synapsin I from a SDS homogenate of 200 μg of rat brain protein.

      Note: The above dilutions are with 35S-protein A; with ECL dilutions may need to be considerably higher to obtain specific immunolabeling.

      Immunohistochemistry:
      1:500-1:2,500

      ELISA:
      1:2,500-1:10,00

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynapsin I (mixture of Ia & Ib) purified from bovine brain.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificitySynapsin I (Mr of 80,000 and 77,000). Synapsin is located only in brain nerve terminal and is an excellent marker for synapes. Immunolabeling is blocked by preadsorption of the antibody with synapsin I.
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
      Gene Symbol
      • SYN1
      • SYN1a
      • SYNI
      • SYN1b
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P17600 # Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
      SIZE: 705 amino acids; 74111 Da
      SUBUNIT: Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1 (By similarity).
      SUBCELLULAR LOCATION: Cell junction, synapse.
      PTM: Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.
      DISEASE: SwissProt: P17600 # Defects in SYN1 are a cause of X-linked recessive epilepsy associated with variable learning disabilities and behavior disorders [MIM:300491].
      SIMILARITY: SwissProt: P17600 ## Belongs to the synapsin family.
      Molecular Weight77 & 80 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceImmunohistochemistry(paraffin):
      Synapsin I (AB1543P) representative staining pattern/morphology in rat hippocampal neurons. Tissue was pretreated with Citrate pH 6.0, antigen retrieval. This lot of antibody was diluted to 1:1000, IHC-Select® reagents used with HRP-DAB. Immunoreactivity is seen directly associated with terminal end of neuronal cell body.
      IHC-Paraffin Staining With Epitope Retrieval:
      Rat Hippocampus
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Synapsin I Antibody MSDS

      职位

      物料安全数据表 (MSDS) 

      Anti-Synapsin I Antibody 分析证书

      标题批号
      Anti-Synapsin I - 2382104 2382104
      Anti-Synapsin I - 2458941 2458941
      Anti-Synapsin I - 2010646 2010646
      Anti-Synapsin I - 2159638 2159638
      Anti-Synapsin I - 2280828 2280828
      Anti-Synapsin I - 2493928 2493928
      Anti-Synapsin I - 3172452 3172452
      Anti-Synapsin I - 3240486 3240486
      Anti-Synapsin I - 3274383 3274383
      Anti-Synapsin I - 3279317 3279317

      参考

      参考概述应用品种公共医疗ID
      A compartmentalized microfluidic neuromuscular co-culture system reveals spatial aspects of GDNF functions.
      Zahavi, EE; Ionescu, A; Gluska, S; Gradus, T; Ben-Yaakov, K; Perlson, E
      Journal of cell science  128  1241-52  2015

      显示摘要
      Immunocytochemistry25632161 25632161
      Structural basis for extracellular cis and trans RPTPσ signal competition in synaptogenesis.
      Coles, CH; Mitakidis, N; Zhang, P; Elegheert, J; Lu, W; Stoker, AW; Nakagawa, T; Craig, AM; Jones, EY; Aricescu, AR
      Nature communications  5  5209  2014

      显示摘要
      25385546 25385546
      Synaptic function is modulated by LRRK2 and glutamate release is increased in cortical neurons of G2019S LRRK2 knock-in mice.
      Beccano-Kelly, DA; Kuhlmann, N; Tatarnikov, I; Volta, M; Munsie, LN; Chou, P; Cao, LP; Han, H; Tapia, L; Farrer, MJ; Milnerwood, AJ
      Frontiers in cellular neuroscience  8  301  2014

      显示摘要
      Immunofluorescence25309331 25309331
      Expression of microRNAs and other small RNAs in prefrontal cortex in schizophrenia, bipolar disorder and depressed subjects.
      Smalheiser, NR; Lugli, G; Zhang, H; Rizavi, H; Cook, EH; Dwivedi, Y
      PloS one  9  e86469  2014

      显示摘要
      24475125 24475125
      Linker mutations reveal the complexity of synaptotagmin 1 action during synaptic transmission.
      Liu, H; Bai, H; Xue, R; Takahashi, H; Edwardson, JM; Chapman, ER
      Nature neuroscience  17  670-7  2014

      显示摘要
      24657966 24657966
      Genetic removal of matrix metalloproteinase 9 rescues the symptoms of fragile X syndrome in a mouse model.
      Sidhu, H; Dansie, LE; Hickmott, PW; Ethell, DW; Ethell, IM
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  9867-79  2014

      显示摘要
      Immunofluorescence25057190 25057190
      Distribution of Na,K-ATPase α subunits in rat vestibular sensory epithelia.
      Schuth, O; McLean, WJ; Eatock, RA; Pyott, SJ
      Journal of the Association for Research in Otolaryngology : JARO  15  739-54  2014

      显示摘要
      ImmunohistochemistryRat25091536 25091536
      Live imaging of endogenous PSD-95 using ENABLED: a conditional strategy to fluorescently label endogenous proteins.
      Fortin, DA; Tillo, SE; Yang, G; Rah, JC; Melander, JB; Bai, S; Soler-Cedeño, O; Qin, M; Zemelman, BV; Guo, C; Mao, T; Zhong, H
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  16698-712  2014

      显示摘要
      Western Blotting25505322 25505322
      Reduction of AP180 and CALM produces defects in synaptic vesicle size and density.
      Petralia, RS; Wang, YX; Indig, FE; Bushlin, I; Wu, F; Mattson, MP; Yao, PJ
      Neuromolecular medicine  15  49-60  2013

      显示摘要
      22851330 22851330
      Microglia actively regulate the number of functional synapses.
      Ji, K; Akgul, G; Wollmuth, LP; Tsirka, SE
      PloS one  8  e56293  2013

      显示摘要
      23393609 23393609

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      Alternative Format

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      种类

      Life Science Research > Antibodies and Assays > Primary Antibodies