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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MABE462 Sigma-AldrichAnti-TET2 Antibody, clone hT2H 21F11

Detect Tet2 using this mouse monoclonal antibody, Anti-TET2 Antibody, clone hT2H 21F11 validated for use in Chromatin IP (ChIP), western blotting & IP.

Anti-TET2 Antibody, clone hT2H 21F11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
技术信息

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: TET2 UniProt Number: Q6N021

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	ChIP, WB, IP	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABE462
Description	Anti-TET2 Antibody, clone hT2H 21F11
Alternate Names	Methylcytosine dioxygenase TET2
Background Information	Methylcytosine dioxygenase TET2 catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). The function of 5-hydroxymethylcytosine is currently unclear but it may influence chromatin structure, or act as an intermediate component in cytosine demethylation. TET2 is frequently mutated in myeloproliferative disorders (MPD) or myeloproliferative neoplasms (MPN), and systemic mastocytosis. TET2 disorders also cause polycythemia vera (PV) and myelodysplastic syndrome (MDS).

References

Product Information
Format	Purified
Control	MOLT-4 cell lysate
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Detect Tet2 using this mouse monoclonal antibody, Anti-TET2 Antibody, clone hT2H 21F11 validated for use in Chromatin IP (ChIP), western blotting & IP.
Key Applications	Chromatin Immunoprecipitation (ChIP) Western Blotting Immunoprecipitation
Application Notes	Western Blotting Analysis: 1.0 µg/mL from a representative lot detected TET2 in 10 µg of TF-1 cell lysate. Immunoprecipitation Analysis: 5 µg of a representative lot immunoprecipitated TET2 in 250 µg of HL60 RIPA cell lysate.

Biological Information
Immunogen	Recombinant protein corresponding to the N-terminus of human TET2.
Epitope	N-terminus
Clone	hT2H21F11
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes the N-terminus of TET2
Isotype	IgG1κ
Species Reactivity	Human Mouse
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001120680
Gene Symbol	TET2
Purification Method	Protein G Purified
UniProt Number	Q6N021
Molecular Weight	~ 260 kDa observed. The calculated molecular weight of this protein is 224 kDa, but can be observed at ~260 kDa.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in MOLT-4 cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected TET2 in 10 µg of MOLT-4 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABE462	04053252911767

Documentation

Anti-TET2 Antibody, clone hT2H 21F11 MSDS

职位
物料安全数据表 (MSDS)

Anti-TET2 Antibody, clone hT2H 21F11 分析证书

标题	批号
Anti-TET2, clone hT2H 21F11	2480977
Anti-TET2, clone hT2H 21F11 - 3218253	3218253
Anti-TET2, clone hT2H 21F11 - 3305921	3305921
Anti-TET2, clone hT2H 21F11 - 3365618	3365618
Anti-TET2, clone hT2H 21F11 - 3560115	3560115
Anti-TET2, clone hT2H 21F11 - 3739776	3739776
Anti-TET2, clone hT2H 21F11 - 3933214	3933214
Anti-TET2, clone hT2H 21F11 - 3933647	3933647
Anti-TET2, clone hT2H 21F11 - 4194302	4194302
Anti-TET2, clone hT2H 21F11 - 4265808	4265808

参考

参考概述	应用	公共医疗ID
PU.1 target genes undergo Tet2-coupled demethylation and DNMT3b-mediated methylation in monocyte-to-osteoclast differentiation. de la Rica, Lorenzo, et al. Genome Biol., 14: R99 (2013) 2013 显示摘要 DNA methylation is a key epigenetic mechanism for driving and stabilizing cell-fate decisions. Local deposition and removal of DNA methylation are tightly coupled with transcription factor binding, although the relationship varies with the specific differentiation process. Conversion of monocytes to osteoclasts is a unique terminal differentiation process within the hematopoietic system. This differentiation model is relevant to autoimmune disease and cancer, and there is abundant knowledge on the sets of transcription factors involved.	Chromatin Immunoprecipitation (ChIP)	24028770

技术信息

标题
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

产品目录编号	描述
ABE364	Anti-Tet2 Antibody

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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