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MABF1110 Anti-UBR5 Antibody, clone 16G10.1

Anti-UBR5, clone 16G10.1, Cat. No. MABF1110, is a mouse monoclonal antibody that detects E3 ubiquitin-protein ligase UBR5 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-UBR5 Antibody, clone 16G10.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: UBR5, EDD, EDD1, HYD, KIAA0896 UniProt Number: O95071
MABF1110
100 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF1110
DescriptionAnti-UBR5 Antibody, clone 16G10.1
Alternate Names
  • E3 ubiquitin-protein ligase UBR5
  • EC: 2.3.2.26
  • E3 ubiquitin-protein ligase
  • HECT domain-containing 1
  • HECT-type E3 ubiquitin transferase UBR5
  • Hyperplastic discs protein homolog
  • hHYD
  • Progestin-induced protein
Background InformationE3 ubiquitin-protein ligase UBR5 (UniProt; O95071; also known as EC: 2.3.2.26, E3 ubiquitin-protein ligase, HECT domain-containing 1, HECT-type E3 ubiquitin transferase UBR5, Hyperplastic discs protein homolog, hHYD, Progestin-induced protein) is encoded by the UBR5 (also known as EDD, EDD1, HYD, KIAA0896) gene (Gene ID: 51366) in human. UBR5 is a widely expressed nuclear E3 ligase that is most abundant in testis and expressed at high levels in brain, pituitary and kidney. It is a component of the N-end rule pathway that recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. UBR5 is involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination and may play a role in control of cell cycle progression. It is shown to regulate DNA topoisomerase II binding protein (TopBP1) in the DNA damage response and may have a tumor suppressive function. It acts as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of Lys-63-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes. URB5 contains a zinc-finger domain (aa 1177-1245), a poly(A)-binding protein C-terminal (PABC) domain(aa 2377-2454), and a (Homologous to the E6-AP Carboxyl Terminus (HECT) domain (aa 2462-2799). Two isoforms of UBR5 have been described that are produced by alternative splicing.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-UBR5, clone 16G10.1, Cat. No. MABF1110, is a mouse monoclonal antibody that detects E3 ubiquitin-protein ligase UBR5 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected UBR5 in human cerebral cortex, human tonsil, and human prostate cancer tissues.
Biological Information
ImmunogenGST/His-tagged recombinant fragment corresponding to 181 amino acids from the C-terminal half of human E3 ubiquitin-protein ligase UBR5.
Clone16G10.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 16G10.1 detects E3 ubiquitin-protein ligage UBR5 in human cells. It targets an epitope within 181 amino acids from the C-terminal half.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • UBR5
  • EDD
  • EDD1
  • HYD
  • KIAA0896
Purification MethodProtein G purified
UniProt Number
Molecular Weight~305 kDa oserved; 309.35 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected UBR5 in 10 µg of Raji cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABF1110 04054839396717