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MABE1796 Anti-YL-1 Antibody, clone EX-1Y32

Anti-YL-1, clone EX-1Y32, Cat. No. MABE1796, is a highly specific mouse monoclonal antibody that targets Vacuolar protein sorting-associated protein 72 homolog and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Anti-YL-1 Antibody, clone EX-1Y32 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: VPS72, TCFL1, YL1 UniProt Number: Q15906
MABE1796
100 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HICC, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1796
DescriptionAnti-YL-1 Antibody, clone EX-1Y32
Alternate Names
  • Vacuolar protein sorting-associated protein 72 homolog
  • Protein YL-1
  • Transcription factor-like 1
Background InformationVacuolar protein sorting-associated protein 72 homolog (UniProt: Q15906; also known as Protein YL-1, Transcription factor-like 1) is encoded by the VPS72 (also known as TCFL1, YL1) gene (Gene ID: 6944) in human. YL-1 is a nuclear protein that may serve as a DNA-binding transcriptional regulator and may be involved in chromatin modification and remodeling. YL-1 is plays multiple roles in chromatin modification and remodeling in cells and it is shown to be a subunit of TRRAP/TIP60 HAT complex and of chromatin remodeling SCCAP-containing complex. It has a DNA binding region that includes amino acids 156 to 206. In addition, it has an aspartic/glutamic acid rich acidic region (aa 22-77) and a proline rich region (aa 333-354). Two isoforms of YL-1 have been described that are produced by alternative splicing. (Ref.: Cai, Y., et al. (2006). J. Biol. Chem. 280 (14); 13665-70).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide and 40% glycerol.
Quality LevelMQ100
Applications
ApplicationAnti-YL-1, clone EX-1Y32, Cat. No. MABE1796, is a highly specific mouse monoclonal antibody that targets Vacuolar protein sorting-associated protein 72 homolog and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected YL-1 in Western Blotting applications (Obri, A., et. al. (2014). Nature. 505(7485):648-53).

Immunocytochemistry Analysis: A 1:800 dilution from a representative lot detected YL-1 in HeLa cells.

Western Blotting Analysis: 2 µg/mL from a representative lot detected YL-1 in 10 µg of HeLa cell lysate.

Immunoprecipitation Analysis: 1-10 µg from a representative lot detected YL-1 in Mouse embryonic fibroblast (MEF) total cell extracts.
Biological Information
ImmunogenGST-tagged full-length recombinant human YL-1 protein.
CloneEX-1Y32
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone EX-1Y32 detects Vacuolar protein sorting-associated protein 72 homolog (YL-1) in human and murine cells.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • VPS72
  • TCFL1
  • YL1
Purification MethodProtein A + Protein G
UniProt Number
Molecular Weight~48 kDa observed, 40.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Mouse embryonic fibroblast (MEF) cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected YL-1 in Mouse embryonic fibroblast (MEF) cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABE1796 04054839352539