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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MABT824 Sigma-AldrichAnti-gamma-Actin/ACTG1 Antibody, clone 2A3

This Anti-gamma-Actin/ACTG1 Antibody, clone 2A3 is validated for use in Western Blotting, Immunocytochemistry for the detection of ACTG1.

Anti-gamma-Actin/ACTG1 Antibody, clone 2A3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: ACTG1, ACTG, BRWS2, DFNA20, DFNA26 UniProt Number: P63261

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, H, Ch, R	WB, ICC	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABT824
Description	Anti-gamma-Actin/ACTG1 Antibody, clone 2A3
Alternate Names	Actin, cytoplasmic 2 gamma-Actin
Background Information	Actin, cytoplasmic 2 (UniProt P63261; also known as Gamma-actin) is encoded by the ACTG1 (also known as ACTG, BRWS2, DFNA20, DFNA26) gene (Gene ID 71) in human. Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin is encoded by the ACTA1 gene, smooth muscle alpha-actin by the ACTA2 gene, cytoplasmic beta-actin by the ACTB gene, cardiac muscle alpha-actin by the ACTC1 gene, cytoplasmic gamma-actin by the ACTG1 gene, and smooth muscle gamma-actin by the ACTG2 (a.k.a. ACTA3) gene. Although actins show >90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins, are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-gamma-Actin/ACTG1 Antibody, clone 2A3 is validated for use in Western Blotting, Immunocytochemistry for the detection of ACTG1.
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Western Blotting Analysis: 0.5 µg/mL from a representative lot detected gamma-Actin/ACTG1 in 10 µg of NIH/3T3 cell lysate. Immunocytochemistry Analysis: 5.0 µg/mL from a representative lot detected gamma-Actin/ACTG1 in HUVECs, HeLa and A431 cells. Western Blotting Analysis: A representative lot detected downregulated cytoplasmic gamma-actin levels in stimulated (by A23187, TRAP-6, TNF, LPS, or IFN-γ) human cerebral microvascular endothelial D3 cells (hCMEC/D3) and their microparticles (MPs) when compared with unstimulated hCMEC/D3 and their MPs (Latham, S.L., et al. (2013). FASEB J. 27(2):672-683). Western Blotting Analysis: A representative lot detected siRNA-mediated downregulation of cytoplasmic gamma-actin in A549 human lung carcinoma cells (Miazza, V., et al. (2011). Virology. 410(1):7-16). Western Blotting Analysis: A representative lot detected cytoplasmic gamma-actin, but not beta-actin separated by 2-D gel electrophoresis of purified chicken gizzard actins or total protein extracts from human subcutaneous fibroblasts (HSCFs), canine MDCK cells, and rat aorta tissue (Dugina, V., et al. (2009). J. Cell Sci. 122(Pt 16):2980-2988). Western Blotting Analysis: A representative lot detected BSA conjugated with cytoplasmic gamma-actin N-terminal peptide, but not BSA conjugated with N-terminal peptides derived from the 5 other actin types (Dugina, V., et al. (2009). J. Cell Sci. 122(Pt 16):2980-2988). Immunocytochemistry Analysis: A representative lot detected TNF-stimulated localization of γ-actin into thick, intensely staining stress fibers concentrated apically in a submembranous network in human cerebral microvascular endothelial D3 cells (hCMEC/D3) (Latham, S.L., et al. (2013). FASEB J. 27(2):672-683). Immunocytochemistry Analysis: A representative lot detected similar γ-actin cytoskeleton structure in human cerebral microvascular endothelial D3 cells (hCMEC/D3) with or without Rho kinase inhibitor Y-27632 treatment (Latham, S.L., et al. (2013). FASEB J. 27(2):672-683). Immunocytochemistry Analysis: A representative lot detected a drastic subcellular redistribution of gamma-actin following Sendai virus infection of polarized Madin-Darby canine kidney (MDCK) epithelial cells by fluorescent immunocytochemistry staining of paraformaldehyde-fixed, methanol-treated cells (Miazza, V., et al. (2011). Virology. 410(1):7-16). Immunocytochemistry Analysis: A representative lot detected cytoplasmic gamma-actin subcellular localization distinct from that of beta-actin in both spreading and stationary cells by fluorescent immunocytochemistry, using paraformaldehyde-fixed, methanol-treated HSCF human subcutaneous fibroblasts, HaCaT human keratinocytes, WI38 human embryonic fibroblasts,and Madin-Darby canine kidney (MDCK) cells (Dugina, V., et al. (2009). J. Cell Sci. 122(Pt 16):2980-2988).

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to the the N-terminal sequence of human gamma-Actin/ACTG1.
Epitope	Near N-terminus.
Clone	2A3
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone 2A3 detected BSA conjugated with cytoplasmic gamma-actin N-terminal peptide, but not BSA conjugated with N-terminal peptides derived from the other 5 actin types (Dugina, V., et al. (2009). J. Cell Sci. 122(Pt 16):2980-2988).
Isotype	IgG2bκ
Species Reactivity	Mouse Human Chicken Rat
Species Reactivity Note	Mouse, Human, Chicken, Rat. Predicted to react with all Mammals based on 100% sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001605.1
Gene Symbol	ACTG1 ACTG BRWS2 DFNA20 DFNA26
Purification Method	Protein G Purified
UniProt Number	P63261
Molecular Weight	~39-45 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected gamma-Actin/ACTG1 in 10 µg of HeLa cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABT824	04055977331165

Documentation

Anti-gamma-Actin/ACTG1 Antibody, clone 2A3 MSDS

职位
物料安全数据表 (MSDS)

Anti-gamma-Actin/ACTG1 Antibody, clone 2A3 分析证书

标题	批号
Anti-gamma-Actin/ACTG1, clone 2A3	3075584
Anti-gamma-Actin/ACTG1, clone 2A3 - 3249915	3249915
Anti-gamma-Actin/ACTG1, clone 2A3 - 3419586	3419586
Anti-gamma-Actin/ACTG1, clone 2A3 - 3709486	3709486
Anti-gamma-Actin/ACTG1, clone 2A3 - 3954773	3954773
Anti-gamma-Actin/ACTG1, clone 2A3 - 4203374	4203374
Anti-gamma-Actin/ACTG1, clone 2A3 - 4290090	4290090
Anti-gamma-Actin/ACTG1, clone 2A3 -Q2653599	Q2653599

产品目录编号	描述
AB3265	Anti-Actin γ Antibody

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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