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ABC505 Anti-hERV-WL Antibody

Detect HERV using this rabbit polyclonal antibody, Anti-hERV-WL Antibody validated for use in western blotting & Flow Cytometry.

Anti-hERV-WL Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: Sec23b UniProt Number: P61561
ABC505
100 µL  
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概述

Replacement Information

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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, FCRbSerumPolyclonal Antibody
Description
Catalogue NumberABC505
DescriptionAnti-hERV-WL Antibody
Alternate Names
  • rWL-1
  • Protein transport protein Sec23B
  • SEC23-related protein B
Background InformationHuman endogenous retrovirus (HERV) proviruses comprise a significant part of the human genome, with approximately 98,000 ERV elements and fragments making up nearly 8% of the DNA. All appear to be inactive containing many deletions and nonsense mutations having integrated into the genome likely millions of years ago but left over HERV DNA as played an active role in genome evolution for millions and years and many important genes expressed today have promoters and enhances that have come from HERV DNA sequences. The best studied example is that of the retroviral fusogenic env proteins. In humans, and other mammals, intact env proteins called syncytins are responsible for the formation and function of syncytiotrophoblasts. These multi-nucleated cells are mainly responsible for maintaining nutrient exchange and protecting the developing fetus from the mother's immune system. The protein HERV-WL is an ancient retroviral envelope (env) protein. This endogenous envelope protein has retained its original fusogenic properties and participates in trophoblast fusion during placenta morphogenesis. HERV-WL gets cleaved into two functional chains upon expression. A surface protein named SU and a transmembrane protein called TM. SU mediates receptor recognition. This interaction triggers the refolding of the transmembrane protein (TM) and is thought to activate its fusogenic potential by unmasking its fusion peptide. The transmembrane protein (TM) acts as a class I viral fusion protein. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of membranes. HERV is expressed during development and in the uterine and placental tissues in the adult.
References
Product Information
FormatSerum
PresentationRabbit polyclonal serum in buffer containing 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect HERV using this rabbit polyclonal antibody, Anti-hERV-WL Antibody validated for use in western blotting & Flow Cytometry.
Key Applications
  • Western Blotting
  • Flow Cytometry
Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected hERV-WL in Jurkat cell lysate.

Flow Cytometry Analysis: 1 µL from a representative lot detected hERV-WL in 1X10E6 Raji and COS-7 cells.
Biological Information
ImmunogenRecombinant protein corresponding to human ERV-WL.
HostRabbit
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Gene Symbol
  • Sec23b
Purification MethodUnpurified
UniProt Number
Molecular Weight~60 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Tera-2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected hERV-WL in 10 µg of Tera-2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
ABC505 04053252937477