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MABC1158 Anti-phospho-MLKL (Ser345) Antibody, clone 7C6.1

Detect phospho MLKL Ser345 using this mouse monoclonal Anti-phospho-MLKL (Ser345), clone 7C6.1, Cat. No. MABC1158. It has been tested in ELISA, Immunocytochemistry, and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: Mlkl UniProt Number: Q9D2Y4
MABC1158
100 µL  
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概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
MICC, WB, ELISAMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1158
DescriptionAnti-phospho-MLKL (Ser345) Antibody, clone 7C6.1
Alternate Names
  • Mixed lineage kinase domain-like protein
Background InformationMixed lineage kinase domain-like protein (UniProt: Q9D2Y4; also known as MLKL) is encoded by the Mlkl gene (Gene ID: 74568) in murine species. MLKL is a pseudokinase that plays a key role in TNF-induced necroptosis. MLKL is highly expressed in thymus, colon, intestine, liver, spleen and lung and is expressed at much lower level in skeletal muscle, heart and kidney. It is not detected in brain. MLKL is activated following phosphorylation by RIPK3 that induces a conformational switch, which is essential for necroptosis. The protein kinase domain of MLKL is catalytically inactive, but contains a pseudoactive site with an interaction between Lys-219 and Gln-343 residues. Upon phosphorylation by RIPK3, MLKL undergoes an active conformation and homotrimerization through its amino-terminal coiled-coil domain. Phosphorylation is also essential for homotrimerization of MLKL and its subsequent localization to the plasma membrane. Plasma membrane localization of MLKL is considered to be essential for Ca2+ influx, which is an early event of TNF-induced necroptosis. (Ref.: Cai, Z et al. (2014). Nat. Cell Biol. 16(1): 55-65).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect phospho MLKL Ser345 using this mouse monoclonal Anti-phospho-MLKL (Ser345), clone 7C6.1, Cat. No. MABC1158. It has been tested in ELISA, Immunocytochemistry, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Western Blotting
  • ELISA
Application NotesELISA Analysis: A representative lot detected phospho-MLKL (Ser345) in ELISA applications (Rodriguez, D.A., et. al. (2016). 23(1):76-88).

Immunocytochemistry Analysis: A representative lot detected phospho-MLKL (Ser345) in Immunocytochemistry applications (Rodriguez, D.A., et. al. (2016). 23(1):76-88).

Western Blotting Analysis: A representative lot detected phospho-MLKL (Ser345) in Western Blotting applications (Rodriguez, D.A., et. al. (2016). 23(1):76-88).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids surrounding Ser345 from the C-terminal half of murine Mixed lineage kinase domain-like protein (MLKL).
Clone7C6.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 7C6.1 specifically detects MLKL phosphorylalated on Ser345. It targets a sequence of 19 amino acids surrounding Ser345 in the C-terminal region.
IsotypeIgG2bκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Mlkl
Modifications
  • Phosphorylation
Purification MethodProtein G purified
UniProt Number
Molecular Weight~54 kDa observed; 54.32 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in MLKL -/- mouse embryonic fibroblasts expressing doxycycline-inducible wild-type MLKL and treated with TNF alpha and Z-VAD-FMK.

Western Blotting Analysis: A 1:4,000 dilution of this antibody detected phospho-MLKL Ser345 in 20 ug of lysate from MLKL -/- mouse embryonic fibroblasts expressing doxycycline-inducible wild-type MLKL-Flag-C construct and treated with TNF alpha (10 ng/mL) and Z-VAD-FMK (25 uM).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABC1158 04054839174469

Documentation

Anti-phospho-MLKL (Ser345) Antibody, clone 7C6.1 MSDS

职位

物料安全数据表 (MSDS) 

Anti-phospho-MLKL (Ser345) Antibody, clone 7C6.1 分析证书

标题批号
Anti-phospho-MLKL (Ser345), clone 7C6.1 - 3471964 3471964
Anti-phospho-MLKL (Ser345), clone 7C6.1 - 3784171 3784171
Anti-phospho-MLKL (Ser345), clone 7C6.1 - 4181453 4181453
Anti-phospho-MLKL (Ser345), clone 7C6.1 - 4250381 4250381
Anti-phospho-MLKL (Ser345), clone 7C6.1 MONOCLONAL ANTIBODY Q2821638
Anti-phospho-MLKL (Ser345), clone 7C6.1 Monoclonal Antibody 3017555
Anti-phospho-MLKL (Ser345), clone 7C6.1 Monoclonal Antibody 2955658
Anti-phospho-MLKL (Ser345), clone 7C6.1 Monoclonal Antibody 3083580
Anti-phospho-MLKL (Ser345), clone 7C6.1 Monoclonal Antibody 2990495
Anti-phospho-MLKL (Ser345), clone 7C6.1 Monoclonal Antibody 3094607
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